Identification, molecular cloning and expression analysis of five RNA-dependent RNA polymerase genes in Salvia miltiorrhiza.
Identification, molecular cloning and expression analysis of five RNA-dependent RNA polymerase genes in Salvia miltiorrhiza.
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丹参中5个RNA依赖性RNA聚合酶基因的鉴定、分子克隆及表达分析
DOI：
10.1371/journal.pone.0095117
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Shao F;Lu S
RNA-dependent RNA polymerases (RDRs) act as key components of the small RNA biogenesis pathways and play significant roles in post-transcriptional gene silencing (PTGS) and antiviral defense. However, there is no information about the RDR gene family in Salvia miltiorrhiza, an emerging model medicinal plant with great economic value. Through genome-wide predication and subsequent molecular cloning, five full-length S. miltiorrhiza RDR genes, termed SmRDR1–SmRDR5, were identified. The length of SmRDR cDNAs varies between 3,262 (SmRDR5) and 4,130 bp (SmRDR3). The intron number of SmRDR genes varies from 3 (SmRDR1, SmRDR3 and SmRDR4) to 17 (SmRDR5). All of the deduced SmRDR protein sequences contain the conserved RdRp domain. Moreover, SmRDR2 and SmRDR4 have an additional RRM domain. Based on the phylogenetic tree constructed with sixteen RDRs from Arabidopsis, rice and S. miltiorrhiza, plant RDRs may be divided into four groups (RDR1–RDR4). The RDR1 group contains an AtRDR and an OsRDR, while includes two SmRDRs. On the contrary, the RDR3 group contains three AtRDRs and two OsRDRs, but has only one SmRDR. SmRDRs were differentially expressed in flowers, leaves, stems and roots of S. miltiorrhiza and responsive to methyl jasmonate treatment and cucumber mosaic virus infection. The results suggest the involvement of RDRs in S. miltiorrhiza development and response to abiotic and biotic stresses. It provides a foundation for further studying the regulation and biological functions of SmRDRs and the biogenesis pathways of small RNAs in S. miltiorrhiza.
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DOI：
10.1016/0005-2787(71)90602-2
发表时间：
1971-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ASTIERMA.S;CORNUET, P
通讯作者：
CORNUET, P
影响因子：
11.6
作者：
Diaz-Pendon, Juan A.;Li, Feng;Ding, Shou-Wei
通讯作者：
Ding, Shou-Wei
影响因子：
3.1
作者：
Lu, Jianli;Sivamani, Elumalai;Qu, Rongda
通讯作者：
Qu, Rongda
影响因子：
4.5
作者：
Moissiard, Guillaume;Parizotto, Eneida Abreu;Voinnet, Olivier
通讯作者：
Voinnet, Olivier
影响因子：
10.3
作者：
He, S.;Yang, Y.;Zhang, X.
通讯作者：
Zhang, X.