Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11
Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11
复制标题
携带来自 MRL/MpJ 型 11 号染色体的同源区域的 C57BL/6N 小鼠出现肾积水伴输尿管炎
DOI：
10.1080/08916934.2016.1261831
复制
发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Y. Kon
中科院分区：
文献类型：
--
作者：
O. Ichii;Masataka Chihara;Shin;Teppei Nakamura;Saori Otsuka;T. Horino;Y. Elewa;Y. Kon
摘要近交系MRL/MpJ小鼠在组织再生过程、泌尿生殖系统和免疫系统中表现出几种独特的表型。阐明这些表型的遗传和分子基础需要分析其遗传易感性位点。在此，在MRL/MpJ衍生的11号染色体（D11 Mit 21 -212）携带C57 BL/6 N的同类小鼠中偶然观察到肾积水的发展，这些小鼠在雄性和雌性中分别以23.5%和12.5%的患病率发展为双侧或单侧肾积水。组织学上，肾盂输尿管交界处移行上皮的乳头状畸形似乎限制了输尿管腔入口。特征性地，在病变输尿管的管腔中观察到嗜酸性晶体。这些输尿管被主要由大量CD 3 + T细胞和B220+ B细胞组成的浸润细胞包围。此外，还检测到几个Iba-1+巨噬细胞、Gr-1+粒细胞、肥大细胞和几丁质酶3样3/Ym 1（一种重要的炎性凝集素）阳性细胞。嗜酸性粒细胞也聚集在病变输尿管的这些病变处。一些B6.MRL-（D11 Mit 21-D11 Mit 212）小鼠具有重复输尿管。我们确定了C57 BL/6 N-和MRL/MpJ-型11号染色体同源区域之间的>100个单核苷酸变异，这些变异与编码蛋白的非同义替换、移码或停止增益有关。总之，B6.MRL-（D11 Mit 21-D11 Mit 212）小鼠由于梗阻性尿路病伴炎症自发发生肾积水。因此，该小鼠系将在实验医学中用于梗阻性尿路病的分子病理学分析。
Abstract Inbred MRL/MpJ mice show several unique phenotypes in tissue regeneration processes and the urogenital and immune systems. Clarifying the genetic and molecular bases of these phenotypes requires the analysis of their genetic susceptibility locus. Herein, hydronephrosis development was incidentally observed in MRL/MpJ-derived chromosome 11 (D11Mit21-212)-carrying C57BL/6N-based congenic mice, which developed bilateral or unilateral hydronephrosis in both males and females with 23.5% and 12.5% prevalence, respectively. Histopathologically, papillary malformations of the transitional epithelium in the pelvic-ureteric junction seemed to constrict the ureter luminal entrance. Characteristically, eosinophilic crystals were observed in the lumen of diseased ureters. These ureters were surrounded by infiltrating cells mainly composed of numerous CD3+ T-cells and B220+ B-cells. Furthermore, several Iba-1+ macrophages, Gr-1+ granulocytes, mast cells and chitinase 3-like 3/Ym1 (an important inflammatory lectin)-positive cells were detected. Eosinophils also accumulated to these lesions in diseased ureters. Some B6.MRL-(D11Mit21-D11Mit212) mice had duplicated ureters. We determined >100 single nucleotide variants between C57BL/6N- and MRL/MpJ-type chromosome 11 congenic regions, which were associated with nonsynonymous substitution, frameshift or stopgain of coding proteins. In conclusion, B6.MRL-(D11Mit21-D11Mit212) mice spontaneously developed hydronephrosis due to obstructive uropathy with inflammation. Thus, this mouse line would be useful for molecular pathological analysis of obstructive uropathy in experimental medicine.
登录
查看更多内容
影响因子：
2.3
作者：
Nio, J;Fujimoto, W;Iwanaga, T
通讯作者：
Iwanaga, T
MRL/MpJ 小鼠卵巢网来源卵巢囊肿发育的数量性状位点分析
DOI：
--
发表时间：
2009
期刊：
影响因子：
--
作者：
Lee;S. -H
通讯作者：
S. -H
MRL/MpJ 小鼠精母细胞热应激抗性的遗传连锁分析。
DOI：
--
发表时间：
2005
期刊：
Mammal. Genome 16(2)
影响因子：
--
作者：
Namiki;Y.
通讯作者：
Y.
影响因子：
19.6
作者：
Ichii, Osamu;Konno, Akihiro;Kon, Yasuhiro
通讯作者：
Kon, Yasuhiro
影响因子：
2.6
作者：
Ichii, O.;Kamikawa, A.;Kon, Y.
通讯作者：
Kon, Y.