A study on cell viability based on thermal inkjet three-dimensional bioprinting
A study on cell viability based on thermal inkjet three-dimensional bioprinting
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基于热喷墨三维生物打印的细胞活力研究
DOI：
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Weixin Fu
中科院分区：
文献类型：
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作者：
Qiushi Wang;Yuan;Y. Ho;Kun Wang;Wenzui Jin;Yimin Guan;Weixin Fu
热喷墨三维（3D）生物打印（TIJ）是一种生物增材制造技术，具有高细胞活力，快速打印速度和低成本。它广泛应用于生物学、化学和制药业。近年来，在使用TIJ打印生物组织方面取得了显著的成果。然而，很少有研究报道TIJ和细胞活力之间的关系。特别地，还没有关于细胞活力和TIJ输入能量的报道。在这项工作中，我们的目标是确定输入脉冲，打印频率和TIJ工作原理的细胞活力之间的关系，并找到一个优化的脉冲波形，以提高细胞活力。我们提出了一种新的方法来研究细胞活力。在控制打印脉冲和频率的同时观察液滴的状态，然后确定相应的细胞存活率。结果表明，脉冲的增加增加了生物墨水中的剪切应力和温度，这降低了细胞的活力。打印细胞的剪切应力和活力显示出相应的分段函数关系。当环境温度高于40 °C时，细胞活力显著降低。增加打印频率会降低打印热损失的速率，从而提高环境温度并损害细胞活力。最后，优化的输入波形可以将细胞活力提高高达约95%。
Thermal inkjet three-dimensional (3D) bioprinting (TIJ) is a biological additive manufacturing technology with high cell viability, fast printing speeds, and low costs. It is widely used in biology, chemistry, and pharmaceuticals. In recent years, remarkable results have been achieved in the printing of biological tissues using TIJ. However, few studies have reported on the relationship between TIJ and cell viability. In particular, there have been no reports relating cell viability and the TIJ input energy. In this work, we aim to determine the relationship between the input pulse, printing frequency, and cell viability from the TIJ working principle and find an optimized pulse waveform to improve cell viability. We propose a novel approach to study cell viability. The state of the droplet is observed while controlling the printing pulse and frequency, and then, the corresponding cell viability is determined. The results show that an increase in the pulse increases the shear stress and temperature in the bio-ink, which reduces the viability of the cells. The shear stress and viability of the printed cells show a corresponding piecewise functional relationship. The cell viability is significantly reduced when the ambient temperature is higher than 40 °C. Increasing the printing frequency reduces the rate of printing heat loss, thereby raising the ambient temperature and impairing cell viability. Finally, the optimized input waveform can increase cell viability by up to about 95%.
影响因子：
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作者：
Cui X;Boland T;D'Lima DD;Lotz MK
通讯作者：
Lotz MK