Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
Thioredoxin from the Indianmeal moth Plodia interpunctella: cloning and test of the allergenic potential in mice.
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DOI：
10.1371/journal.pone.0042026
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Duchêne M
中科院分区：
文献类型：
--
作者：
Hoflehner E;Binder M;Hemmer W;Mahler V;Panzani RC;Jarisch R;Wiedermann U;Duchêne M
印度谷螟Plodia interpunctella是人类住宅中高度流行的食物害虫，并且已被证明含有许多过敏原。到目前为止，只有一个，精氨酸激酶（Plo i 1）已被确定。本研究的目的是鉴定进一步的过敏原，并与Plo i 1进行比较。用室内过敏患者血清和蛾类IgE筛选了完整成虫的cDNA文库，并鉴定了硫氧还蛋白为IgE结合蛋白。重组硫氧还蛋白在E.大肠杆菌，并与Plo i 1和全蛾提取物一起在针对大量室内过敏患者血清的IgE免疫印迹中进行测试。用重组硫氧还蛋白和Plo i 1免疫BALB/c小鼠，并测量抗体产生、RBL细胞介体释放、T细胞增殖和细胞因子产生。这是第一次从一个动物物种的硫氧还蛋白被确定为过敏原。约8%的来自具有针对蛾提取物的IgE的患者的血清与重组间点疟原虫硫氧还蛋白反应，相比之下，25%与重组Plo 11反应。在免疫BALB/c小鼠中，重组变应原均诱导经典的Th 2偏向性免疫应答，如诱导IgE和IgG 1抗体，上调IL-5和IL-4以及嗜碱性粒细胞脱粒。来自蛾类如Plo i 1的硫氧还蛋白像来自小麦或玉米的硫氧还蛋白一样充当经典的I型过敏原。这清楚地支持硫氧还蛋白的泛过敏原性质。建议将新的P. interpunctella变应原命名为Plo i 2。
The Indianmeal moth Plodia interpunctella is a highly prevalent food pest in human dwellings, and has been shown to contain a number of allergens. So far, only one of these, the arginine kinase (Plo i 1) has been identified. The aim of this study was to identify further allergens and characterise these in comparison to Plo i 1. A cDNA library from whole adult P. interpunctella was screened with the serum of a patient with indoor allergy and IgE to moths, and thioredoxin was identified as an IgE-binding protein. Recombinant thioredoxin was generated in E. coli, and tested together with Plo i 1 and whole moth extracts in IgE immunoblots against a large panel of indoor allergic patients' sera. BALB/c mice were immunised with recombinant thioredoxin and Plo i 1, and antibody production, mediator release from RBL cells, T-cell proliferation and cytokine production were measured. For the first time a thioredoxin from an animal species was identified as allergen. About 8% of the sera from patients with IgE against moth extracts reacted with recombinant P. interpunctella thioredoxin, compared to 25% reacting with recombinant Plo i 1. In immunised BALB/c mice, the recombinant allergens both induced classical Th2-biased immune responses such as induction IgE and IgG1 antibodies, upregulation of IL-5 and IL-4 and basophil degranulation. Thioredoxin from moths like Plo i 1 acts like a classical Type I allergen as do the thioredoxins from wheat or corn. This clearly supports the pan-allergen nature of thioredoxin. The designation Plo i 2 is suggested for the new P. interpunctella allergen.
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影响因子：
4.6
作者：
Hufnagl, K;Wagner, B;Weidermann, U
通讯作者：
Weidermann, U
影响因子：
12.4
作者：
Komase, Y;Sakata, M;Nakagawa, T
通讯作者：
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影响因子：
4.4
作者：
Limacher, Andreas;Glaser, Andreas G.;Crameri, Reto
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影响因子：
5.8
作者：
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通讯作者：
Higgins, D. G.
DOI：
10.1073/pnas.85.8.2444
发表时间：
1988-04-01
影响因子：
11.1
作者：
PEARSON, WR;LIPMAN, DJ
通讯作者：
LIPMAN, DJ