Patterns of glutamate, glycine, and GABA immunolabeling in four synaptic terminal classes in the lateral superior olive of the guinea pig
Patterns of glutamate, glycine, and GABA immunolabeling in four synaptic terminal classes in the lateral superior olive of the guinea pig
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豚鼠外侧上橄榄四种突触末端类别的谷氨酸、甘氨酸和 GABA 免疫标记模式
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
R. Altschuler
中科院分区：
文献类型：
--
作者：
R. Helfert;J. Juiz;S. Bledsoe;J. M. Bonneau;R. Wenthold;R. Altschuler
本研究的目的是关联突触超微结构与发射机的特异性和功能，在外侧上级橄榄（LSO），一个核，被认为是在声音定位中发挥重要作用。这是通过植入后免疫金免疫细胞化学完成的。在LSO中鉴定出四类突触终末。它们在形态学上和基于它们对谷氨酸、甘氨酸和γ-氨基丁酸（GABA）的不同免疫标记模式彼此可区分。在含有圆形囊泡（R）并与不对称突触连接形成突触接触的终末中发现最高水平的谷氨酸免疫反应性。圆形囊泡终末在小口径树突上占主导地位，其比例至少为2：1。最薄的树突通常仅与R末端接触。R终末与其他类型的比率随着它们并列的树突状轮廓的口径增加而降低，使得在索马上，R终末的数量超过其他类型至少2：1。含有扁平囊泡的末端（F）对甘氨酸和谷氨酸都表现出强烈的免疫反应性，尽管谷氨酸的免疫标记不如R末端中的高。扁平囊泡终末与其靶形成对称的突触接触，其分布与R终末所述的相反;即，在LSO胞体上最多，在小口径树突上最少。两个终端类型，都含有多形性囊泡和形成对称的突触连接，发现在数量少得多。一组含有大的多形性囊泡（LP）和免疫反应的甘氨酸和GABA。另一组含有小的多形性囊泡（SP），沿着有一些致密核心囊泡，仅标记为GABA。LP终末主要分布在胞体和大口径树突上，SP终末主要分布在小树突上。以前的工作表明，很大比例的R终端出现在同侧耳蜗核的球形细胞和兴奋性的行动。这条通路可能使用谷氨酸作为递质。许多的F终端被认为是起源于同侧内侧核的斜方体，似乎是抑制（甘氨酸能）终端从一个途径，起源于对侧耳。LP和SP终端的起源和功能是未知的，但一些可能性进行了讨论沿着的意义上的cocontainment的神经活性物质在特定的终端类型。© 1992 Wiley利斯公司
The goal of this study was to correlate synaptic ultrastructure with transmitter specificity and function in the lateral superior olive (LSO), a nucleus that is thought to play a major role in sound localization. This was accomplished by means of postembedding immunogold immunocytochemistry. Four classes of synaptic terminals were identified in the LSO. They were distinguishable from one another both morphologically and on the basis of their different patterns of immunolabeling for glutamate, glycine, and γ‐aminobutyric acid (GABA). The highest level of glutamate immunoreactivity was found in terminals that contained round vesicles (R) and formed synaptic contacts with asymmetric synaptic junctions. Round‐vesicle terminals predominated on small caliber dendrites by a ratio of at least 2:1 over the other classes combined. The thinnest dendrites were typically contacted by R terminals only. The ratio of R terminals to the other types decreased as the caliber of the dendritic profiles they apposed increased so that on the soma, R terminals were outnumbered by at least 2:1 by the other types. Terminals containing flattened vesicles (F) exhibited intense immunoreactivity for both glycine and glutamate, although the glutamate immunolabeling was not as high as that in the R terminals. Flattened‐vesicle terminals formed symmetric synaptic contacts with their targets and their distribution was the reverse of that described for R terminals; i.e., they were most abundant on LSO perikarya and fewest on small caliber dendrites. Two terminal types, both containing pleomorphic vesicles and forming symmetric synaptic junctions, were found in far fewer numbers. One group contained large pleomorphic vesicles (LP) and was immunoreactive for both glycine and GABA. The other group contained small pleomorphic vesicles (SP) along with a few dense‐core vesicles and labeled for GABA only. The LP terminals were preferentially distributed on somata and large–caliber dendrites, while the SP terminals most often contacted smaller dendrites. Previous work suggests that a large percentage of the R terminals arise from spherical cells in the ipsilateral cochlear nucleus and are excitatory in action. This pathway may use glutamate as a transmitter. Many of the F terminals are thought to originate from the ipsilateral medial nucleus of the trapezoid body and appear to be the inhibitory (glycinergic) terminals from a pathway that originates from the contralateral ear. The origins and functions of LP and SP terminals are unknown, but a few possibilities are discussed along with the significance of cocontainment of neuroactive substances in specific terminal types. © 1992 Wiley‐Liss, Inc.
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DOI：
10.1002/jemt.1060150107
发表时间：
1990-05
期刊：
Journal of electron microscopy technique
影响因子：
--
作者：
B. Maley
通讯作者：
B. Maley
豚鼠从耳蜗核到上橄榄的摄取和逆行转运[3H]GABA。
影响因子：
2.8
作者：
Ostapoff,EM;Morest,DK;Potashner,SJ
通讯作者：
Potashner,SJ
正常和传入神经缺失的小脑皮层中谷氨酸和 GABA 的免疫金电子显微镜演示：递质含量与突触小泡大小之间的相关性。
DOI：
10.1177/38.12.1979341
发表时间：
1990
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Hámori,J;Takács,J;Petrusz,P
通讯作者：
Petrusz,P
影响因子：
3.2
作者：
BEITZ, AJ
通讯作者：
BEITZ, AJ
碳二亚胺固定谷氨酸特异性单克隆抗体：大鼠中枢神经系统中的免疫细胞化学定位。
DOI：
10.1177/34.3.2419390
发表时间：
1986
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Madl,JE;Larson,AA;Beitz,AJ
通讯作者：
Beitz,AJ