D(2) receptors receive paracrine neurotransmission and are consistently targeted to a subset of synaptic structures in an identified neuron of the crustacean stomatogastric nervous system.
D(2) receptors receive paracrine neurotransmission and are consistently targeted to a subset of synaptic structures in an identified neuron of the crustacean stomatogastric nervous system.
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DOI：
10.1002/cne.22225
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发表时间：
2010-02-01
期刊：
影响因子：
--
通讯作者：
Baro DJ
中科院分区：
文献类型：
--
作者：
Oginsky MF;Rodgers EW;Clark MC;Simmons R;Krenz WD;Baro DJ
多巴胺(DA)调节从线虫和节肢动物到脊索动物的各种运动系统。在广泛的系统发育范围内对多巴胺能系统进行比较，应该会揭示共同的组织原则。幽门网络位于口胃神经节(STG)，是运动网络神经调节的重要模型。DA对这个网络的影响已经在刺龙虾的回路和细胞水平上得到了很好的表征。在这里，我们提供了关于STG中DA信号系统的物理组织和幽门神经元中D2受体的功能的第一个数据。以前的研究表明，DA改变了幽门扩张器(PD)神经元的固有放电特性和突触输出，部分是通过减少钙电流和增加外向钾电流来实现的。我们进行了单细胞逆转录聚合酶链式反应实验，结果显示帕金森病神经元仅表达2型(D2αPAN)DA受体。使用共聚焦显微镜结合免疫组织化学(IHC)对含有染料填充的PD神经元的STG整装标本进行了证实。免疫金电镜显示，突触神经纤维表面受体主要集中在细小的突起/终末肿胀和充满囊泡的曲张中。用酪氨酸羟化酶抗血清进行双标记免疫组化实验表明，D2αPAN受体接受了容量神经传递。受体被进一步映射到帕金森病神经元的三维模型上，该模型是由IHC实验中共焦堆叠的神经荧光素追踪建立的。结果表明，D2αPAN受体选择性地定位于PD神经元中约40%的突触结构，且分布不均匀。
Dopamine (DA) modulates motor systems in phyla as diverse as nematodes and arthropods up through chordates. A comparison of dopaminergic systems across a broad phylogenetic range should reveal shared organizing principles. The pyloric network, located in the stomatogastric ganglion (STG), is an important model for neuromodulation of motor networks. The effects of DA on this network have been well characterized at the circuit and cellular levels in the spiny lobster, Panulirus interruptus. Here we provide the first data about the physical organization of the DA signaling system in the STG and the function of D2 receptors in pyloric neurons. Previous studies showed that DA altered intrinsic firing properties and synaptic output in the pyloric dilator (PD) neuron, in part by reducing calcium currents and increasing outward potassium currents. We performed single cell reverse transcriptase-polymerase chain reaction (RT-PCR) experiments to show that PD neurons exclusively expressed a type 2 (D2αPan) DA receptor. This was confirmed by using confocal microscopy in conjunction with immunohistochemistry (IHC) on STG whole-mount preparations containing dye-filled PD neurons. Immunogold electron microscopy showed that surface receptors were concentrated in fine neurites/terminal swellings and vesicle-laden varicosities in the synaptic neuropil. Double-label IHC experiments with tyrosine hydroxylase antiserum suggested that the D2αPan receptors received volume neurotransmissions. Receptors were further mapped onto three-dimensional models of PD neurons built from Neurolucida tracings of confocal stacks from the IHC experiments. The data showed that D2αPan receptors were selectively targeted to approximately 40% of synaptic structures in any given PD neuron, and were nonuniformly distributed among neurites.
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影响因子：
5.3
作者：
Baro, DJ;Ayali, A;Harris-Warrick, RM
通讯作者：
Harris-Warrick, RM
影响因子：
2.5
作者：
Cleland, TA;Selverston, AI
通讯作者：
Selverston, AI
DOI：
10.1016/j.cbpb.2006.08.018
发表时间：
2007-01-01
影响因子：
2.2
作者：
Clark, Merry C.;Baro, Deborah J.
通讯作者：
Baro, Deborah J.
影响因子：
7.6
作者：
Bertran-Gonzalez, Jesus;Hakansson, Kerstin;Fisone, Gilberto
通讯作者：
Fisone, Gilberto
影响因子：
2.9
作者：
BARKER, DL;KUSHNER, PD;HOOPER, NK
通讯作者：
HOOPER, NK