Electrophoresis on uncrosslinked polyacrylamide: Molecular sieving and its potential applications
Electrophoresis on uncrosslinked polyacrylamide: Molecular sieving and its potential applications
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非交联聚丙烯酰胺电泳：分子筛分及其潜在应用
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
A. Chrambach
中科院分区：
文献类型：
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作者：
D. Tietz;M. Gottlieb;J. S. Fawcett;A. Chrambach
非交联聚丙烯酰胺是一种粘性液体，在电泳中提供了与交联凝胶相同的分子筛效应。这一发现证实了其他人使用未交联聚丙烯酰胺与各种凝胶基质混合的先前结果。它提供了一种筛分介质，由惰性纤维的完全和必要的随机缔合构成，正如Ogston理论解释迁移所规定的那样。在未交联的聚丙烯酰胺中的几种蛋白质的Ferguson图显示出比在5%交联的凝胶中更小的Yo值和彼此更少的偏离，表明交联的聚丙烯酰胺凝胶的Ferguson图在低%T下的曲率。如果可以证明这些图在低%T、0% C下的线性，则可以通过将其线性外推至0凝胶浓度来确定自由迁移率的准确值。潜在地，低浓度的未交联聚丙烯酰胺可以提供用于亚细胞颗粒的分析筛选的工具。然而，浓度低于10%的未交联聚丙烯酰胺中的电泳会受到对流的干扰，因此这些潜在的应用将取决于将其与合适的抗对流装置组合。
Uncrosslinked polyacrylamide, a viscous liquid, provides a molecular sieving effect in electrophoresis qualitatively the same as that of crosslinked gels. This finding confirms previous results by others using uncrosslinked polyacrylamide mixed with various gel matrices. It provides a sieving medium constituted by an entirely and necessarily random association of inert fibers, as stipulated for interpretation of migration by the Ogston theory. Ferguson plots of several proteins in uncrosslinked polyacrylamide exhibit Yo values smaller and less divergent from one another than in 5 % crosslinked gels, suggesting a curvature of the Ferguson plots of crosslinked polyacrylamide gels at low %T. If linearity of these plots at low %T, 0 %C could be demonstrated, a means would be found to determine accurate values of free mobility by their linear extrapolation to 0 gel concentration. Potentially, uncrosslinked polyacrylamide at low concentrations may provide a tool for the analytical sieving of subcellular particles. However, electrophoresis in uncrosslinked polyacrylamide at concentrations of less than 10 % is perturbed by convection, and thus these potential applications will depend on combining it with a suitable anticonvective device.
蛋白质和核酸在缺乏共价交联的丙烯酰胺-琼脂糖凝胶上的电泳。
影响因子：
2.9
作者：
Horowitz,PM;Lee,JC;Williams,GA;Williams,RF;Barnes,LD
通讯作者：
Barnes,LD