Insertion and excision of Caenorhabditis elegans transposable element Tc1.
Insertion and excision of Caenorhabditis elegans transposable element Tc1.
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秀丽隐杆线虫转座元件 Tc1 的插入和切除。
DOI：
10.1128/mcb.8.2.737-746.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Anderson,P
中科院分区：
文献类型：
--
作者：
Eide,D;Anderson,P
转座元件Tc1是导致优雅线虫Bergerac中大多数自发突变的原因。我们研究了Tc1转座和切除的遗传和分子特性。我们发现Tc1插入UNC-54肌球蛋白重链基因具有很强的位点特异性。相互独立的Tc1插入位点的DNA序列彼此相似，我们给出了描述这些相似之处的Tc1插入的共同序列。我们发现Tc1切除通常是不精确的。Tc1的切除在生殖系和体细胞中都是不精确的。不精确的切除产生了新的c-54等位基因，这些等位基因有氨基酸替换、氨基酸插入，在某些情况下，可能改变了mRNA的剪接。Tc1体细胞切除后残留的DNA序列与胚系切除后的DNA序列相同，但体细胞切除的频率至少是生殖系切除的1000倍。Tc1切除的遗传特性，结合产生的unc-54等位基因的DNA序列，表明Tc1切除取决于Tc1在生殖系和体细胞中的转座功能。与胚系切除不同，体细胞切除不是以相同的品系特定方式进行调节。在胚系中没有检测到Tc1转座和切除的遗传背景下，胞体中的Tc1切除仍然发生在高频上。
The transposable element Tc1 is responsible for most spontaneous mutations that occur inCaenorhabditis elegansvariety Bergerac. We investigated the genetic and molecular properties of Tc1 transposition and excision. We show that Tc1 insertion into the unc-54 myosin heavy-chain gene was strongly site specific. The DNA sequences of independent Tc1 insertion sites were similar to each other, and we present a consensus sequence for Tc1 insertion that describes these similarities. We show that Tc1 excision was usually imprecise. Tc1 excision was imprecise in both germ line and somatic cells. Imprecise excision generated novelunc-54alleles that had amino acid substitutions, amino acid insertions, and, in certain cases, probably altered mRNA splicing. The DNA sequences remaining after Tc1 somatic excision were the same as those remaining after germ line excision, but the frequency of somatic excision was at least 1,000-fold higher than that of germ line excision. The genetic properties of Tc1 excision, combined with the DNA sequences of the resultingunc-54alleles, demonstrated that excision was dependent on Tc1 transposition functions in both germ line and somatic cells. Somatic excision was not regulated in the same strain-specific manner as germ-line excision was. In a genetic background where Tc1 transposition and excision in the germ line was not detectable, Tc1 excision in the soma still occurred at high frequency.
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影响因子：
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作者：
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DOI：
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影响因子：
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影响因子：
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DOI：
10.1073/pnas.80.12.3585
发表时间：
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影响因子：
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