hobo enhancer trapping mutagenesis in Drosophila reveals an insertion specificity different from P elements.
hobo enhancer trapping mutagenesis in Drosophila reveals an insertion specificity different from P elements.
复制标题
hobo 增强子在果蝇中捕获诱变揭示了与 P 元件不同的插入特异性。
作者:
Desmond Smith;Jay Wohlgemuth;Brian R. Calvi;Ian Franklint;William;Gelbart
P element enhancer trapping has become an indispensable tool in the analysis of the Drosophila melanogaster genome. However, there is great variation in the mutability of loci by these elements such that some loci are relatively refractory to insertion. We have developed the hobo transposable element for use in enhancer trapping and we describe the results of a hobo enhancer trap screen. In addition, we present evidence that a hobo enhancer trap element has a pattern of insertion into the genome that is different from the distribution of P elements in the available database. Hence, hobo insertion may facilitate access to genes resistant to P element insertion.
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影响因子:
11.1
作者:
D. Rio
通讯作者:
D. Rio
影响因子:
3.3
作者:
Berg,CA;Spradling,AC
通讯作者:
Spradling,AC
DOI:
10.1073/pnas.83.3.735
发表时间:
1986-02-01
影响因子:
11.1
作者:
SEIFERT, HS;CHEN, EY;HEFFRON, F
通讯作者:
HEFFRON, F
影响因子:
3.3
作者:
P. Athma;Erich Grotewold;Thomas Peterson
通讯作者:
P. Athma;Erich Grotewold;Thomas Peterson
影响因子:
3.3
作者:
Zhang,P;Spradling,AC
通讯作者:
Spradling,AC