Impact of international travel and diarrhea on gut microbiome and resistome dynamics.
Impact of international travel and diarrhea on gut microbiome and resistome dynamics.
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DOI：
10.1038/s41467-022-34862-w
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发表时间：
2022-12-05
影响因子：
16.6
通讯作者：
Dantas, Gautam
中科院分区：
文献类型：
--
作者：
Boolchandani, Manish;Blake, Kevin S.;Tilley, Drake H.;Cabada, Miguel M.;Schwartz, Drew J.;Patel, Sanket;Morales, Maria Luisa;Meza, Rina;Soto, Giselle;Isidean, Sandra D.;Porter, Chad K.;Simons, Mark P.;Dantas, Gautam
国际旅行促进了抗菌素耐药性的全球传播。旅行者的腹泻加剧了感染耐多药微生物的风险，并可能导致旅行后持续的胃肠功能障碍。然而，关于腹泻对旅行者肠道微生物群的影响，以及这些变化在整个旅行过程中的动态，人们知之甚少。在这里，我们召集了159名访问秘鲁安第斯城市库斯科的国际学生，并将下一代测序技术应用于718个纵向收集的粪便样本。我们发现，在整个旅行过程中，肠道微生物群组成发生了显著变化，但分类多样性保持稳定。然而，腹泻破坏了这种稳定性，导致了更多的抗菌素耐药基因，这些基因可以在数周内保持在较高水平。我们还在腹泻性和非腹泻性样本之间确定了不同丰富的分类群，用于开发区分这些疾病状态的分类模型。此外，我们对212株致腹泻大肠杆菌的基因组进行了测序，发现那些来自经历过腹泻的旅行者的菌株比那些没有经历过腹泻的旅行者编码了更多的抗菌素耐药基因。在这项工作中，我们发现国际旅行者的肠道微生物群对生态失调具有弹性；然而，它们也容易受到多重耐药细菌的定居，这一风险在患有腹泻的旅行者中更为明显。在这里，作者描述了159名访问秘鲁安第斯城市库斯科的国际学生的纵向队列中的微生物群和耐药群。他们发现，国际旅行与抗菌素耐药性的传播有关，旅行者的腹泻增加了人们获得抗菌素抗药性病原体的风险，而多样化的“健康”微生物群可以预防腹泻。
International travel contributes to the global spread of antimicrobial resistance. Travelers’ diarrhea exacerbates the risk of acquiring multidrug-resistant organisms and can lead to persistent gastrointestinal disturbance post-travel. However, little is known about the impact of diarrhea on travelers’ gut microbiomes, and the dynamics of these changes throughout travel. Here, we assembled a cohort of 159 international students visiting the Andean city of Cusco, Peru and applied next-generation sequencing techniques to 718 longitudinally-collected stool samples. We find that gut microbiome composition changed significantly throughout travel, but taxonomic diversity remained stable. However, diarrhea disrupted this stability and resulted in an increased abundance of antimicrobial resistance genes that can remain high for weeks. We also identified taxa differentially abundant between diarrheal and non-diarrheal samples, which were used to develop a classification model that distinguishes between these disease states. Additionally, we sequenced the genomes of 212 diarrheagenic Escherichia coli isolates and found those from travelers who experienced diarrhea encoded more antimicrobial resistance genes than those who did not. In this work, we find the gut microbiomes of international travelers’ are resilient to dysbiosis; however, they are also susceptible to colonization by multidrug-resistant bacteria, a risk that is more pronounced in travelers with diarrhea. Here, the authors characterize the microbiome and resistome in a longitudinal cohort of 159 international students visiting the Andean city of Cusco, Peru. They find that international travel associates with spread of antimicrobial resistance, and that travelers’ diarrhea increases a persons’ risk for acquiring antimicrobial-resistant pathogens, while a diverse, “healthy” microbiome can be protective against diarrhea.
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影响因子：
19
作者：
Fernandes, M. R.;Moura, Q.;Lincopan, N.
通讯作者：
Lincopan, N.
DOI：
10.1126/science.1220761
发表时间：
2012-08-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Forsberg KJ;Reyes A;Wang B;Selleck EM;Sommer MO;Dantas G
通讯作者：
Dantas G
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
4.9
作者：
Bengtsson-Palme, Johan;Angelin, Martin;Johanssone, Anders
通讯作者：
Johanssone, Anders
影响因子：
30.3
作者：
Brown, Eric M.;Ke, Xiaobo;Xavier, Ramnik J.
通讯作者：
Xavier, Ramnik J.