Quasispecies evolution of the prototypical genotype 1 porcine reproductive and respiratory syndrome virus early during in vivo infection is rapid and tissue specific.
Quasispecies evolution of the prototypical genotype 1 porcine reproductive and respiratory syndrome virus early during in vivo infection is rapid and tissue specific.
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DOI：
10.1007/s00705-017-3342-0
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发表时间：
2017-08
影响因子：
2.7
通讯作者：
Frossard JP
中科院分区：
文献类型：
--
作者：
Lu ZH;Wang X;Wilson AD;Dorey-Robinson DLW;Archibald AL;Ait-Ali T;Frossard JP
猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus，PRRSV）是危害养猪业的主要传染病之一。越来越多的证据表明，准种群体内的微进化可以引起PRRSV的高序列异质性，可能影响病毒的致病性。在这里，我们报告的微观进化事件发生在肺和淋巴结感染后3天（dpi）的病毒准种人口的原型莱利斯塔PRRSV（LV）的实验体内感染。序列分析揭示了16个高频单核苷酸变异体（SNV）或与参考LV基因组的差异，这些变异体或差异被认为是共有接种物基因组的代表。此外，在接种群体中还发现了49个其他低频SNV。在3dpi时，在感染肺和淋巴结的LV群体中已经分别检测到总共9个和10个不同频率的SNV。有趣的是，在这些中，当与接种物相比时，三种和四种新的SNV独立地出现在两种相应的组织中。其余的变种，虽然已经存在于接种物中的频率较低，积极选择和准种人口内的频率增加。因此，我们能够直接从感染PRRSV的组织中确定病毒准种群体中的遗传变异库。我们的数据还表明，这些变体的微进化是快速的，有些可能是组织特异性的。本文的在线版本（doi：10.1007/s 00705 -017-3342-0）包含补充材料，可供授权用户使用。
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major infectious threat to the pig industry worldwide. Increasing evidence suggests that microevolution within a quasispecies population can give rise to high sequence heterogeneity in PRRSV; potentially impacting the pathogenicity of the virus. Here, we report on micro-evolutionary events taking place within the viral quasispecies population in lung and lymph node 3 days post infection (dpi) following experimental in vivo infection with the prototypical Lelystad PRRSV (LV). Sequence analysis revealed 16 high frequency single nucleotide variants (SNV) or differences from the reference LV genome which are assumed to be representative of the consensus inoculum genome. Additionally, 49 other low frequency SNVs were also found in the inoculum population. At 3 dpi, a total of 9 and 10 SNVs of varying frequencies could already be detected in the LV population infecting the lung and lymph nodes, respectively. Interestingly, of these, three and four novel SNVs emerged independently in the two respective tissues when compared to the inoculum. The remaining variants, though already present at lower frequencies in the inoculum, were positively selected and their frequency increased within the quasispecies population. Hence, we were able to determine directly from tissues infected with PRRSV the repertoire of genetic variants within the viral quasispecies population. Our data also suggest that microevolution of these variants is rapid and some may be tissue-specific. The online version of this article (doi:10.1007/s00705-017-3342-0) contains supplementary material, which is available to authorized users.
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影响因子：
5
作者：
Lu ZH;Archibald AL;Ait-Ali T
通讯作者：
Ait-Ali T
影响因子：
--
作者：
Brown, Elizabeth;Lawson, Steven;Fang, Ying
通讯作者：
Fang, Ying
影响因子：
3.3
作者：
Morgan, S. B.;Graham, S. P.;Frossard, J. P.
通讯作者：
Frossard, J. P.
影响因子：
5
作者：
Kvisgaard, Lise K.;Hjulsager, Charlotte K.;Larsen, Lars E.
通讯作者：
Larsen, Lars E.
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng