Co-culture of neurons and glia in a novel microfluidic platform.
Co-culture of neurons and glia in a novel microfluidic platform.
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DOI：
10.1016/j.jneumeth.2010.12.024
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发表时间：
2011-03-15
影响因子：
3
通讯作者：
Webb, Donna J.
中科院分区：
文献类型：
--
作者：
Majumdar, Devi;Gao, Yandong;Li, Deyu;Webb, Donna J.
在这项研究中，我们开发了一个微流控细胞共培养平台，允许单独操纵不同细胞类型的微环境。细胞培养室的分离通过交替微制造阀的位置来控制，所述微制造阀用作每个室之间的屏障。我们平台的这一独特功能使我们能够在最佳条件下维持海马神经元和神经胶质细胞的健康共培养数周。细胞培养室之间的受控流体交换为神经元提供了持续供应的原位条件神经胶质培养基，这对它们的生存至关重要。使用屏障阀，我们分别用绿色荧光蛋白（GFP）和mCherry cDNA转染相邻室中的神经元，转染效率约为40%。与胶质细胞共培养进一步提高了神经元的转染效率，几乎达到60%。因此，微流控装置为中枢神经系统细胞的长期培养、转染和个体化治疗提供了新的平台。
In this study, we developed a microfluidic cell co-culture platform that permits individual manipulation of the microenvironment of different cell types. Separation of the cell culture chambers is controlled by alternating the position of a microfabricated valve, which serves as a barrier between each chamber. This unique feature of our platform allowed us to maintain healthy co-cultures of hippocampal neurons and glia for several weeks under optimal conditions. Controlled fluidic exchange between the cell culture chambers provided neurons with a continuous supply of in situ conditioned glia media that was critical for their survival. Using the barrier valve, we transfected neurons in the adjacent chambers with green fluorescent protein (GFP) and mCherry cDNA, respectively, with a transfection efficiency of approximately 40%. Co-culture with glia further enhanced the transfection efficiency of neurons to almost 60%. Thus, the microfluidic devices offer a novel platform for the long-term culture, transfection, and individual treatment of central nervous system cells.
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星形胶质细胞兴奋性神经递质转运蛋白 GLT1 的突触前调节。
影响因子：
16.2
作者：
Yang, Yongjie;Gozen, Oguz;Watkins, Andrew;Lorenzini, Ileana;Lepore, Angelo;Gao, Yuanzheng;Vidensky, Svetlana;Brennan, Jean;Poulsen, David;Park, Jeong Won;Jeon, Noo Li;Robinson, Michael B.;Rothstein, Jeffrey D.
通讯作者：
Rothstein, Jeffrey D.
影响因子：
3.9
作者：
Taylor, AM;Rhee, SW;Jeon, NL
通讯作者：
Jeon, NL
影响因子：
6.1
作者：
Hosmane, Suneil;Yang, In Hong;Venkatesan, Arun
通讯作者：
Venkatesan, Arun
DOI：
10.1021/la8021479
发表时间：
2008-11-18
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Chang WC;Sretavan DW
通讯作者：
Sretavan DW
影响因子：
3
作者：
Ravula, Surendra K.;Wang, Min S.;Frazier, A. Bruno
通讯作者：
Frazier, A. Bruno