Increased activated regulatory T cells proportion correlate with the severity of idiopathic pulmonary fibrosis.
Increased activated regulatory T cells proportion correlate with the severity of idiopathic pulmonary fibrosis.
复制标题
活化的调节性 T 细胞比例增加与特发性肺纤维化的严重程度相关
DOI：
10.1186/s12931-017-0653-3
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发表时间：
2017-09-08
影响因子：
5.8
通讯作者：
Zeng H
中科院分区：
文献类型：
--
作者：
Hou Z;Ye Q;Qiu M;Hao Y;Han J;Zeng H
调节性T细胞（Tregs）在维持免疫耐受和免疫稳态中至关重要，但它们在特发性肺纤维化（IPF）中的作用尚不清楚。本研究旨在探讨Tregs在IPF中的作用。用流式细胞术检测29例IPF患者、19例原发性Sjögren综合征相关性间质性肺炎（pSS-IP）患者和23例健康对照（hc）外周血（PB）和支气管肺泡灌洗液（BAL）样本中Tregs及其亚群的百分比。在外周血中，IPF、pSS-IP或hc患者的CD4+CD25+Foxp3+ Treg百分比没有差异。然而，与pSS-IP或hc相比，IPF中CD4+ T细胞中活化的Treg （aTreg）含量显著增加。与PB的结果一致，IPF中CD4+ T细胞中的aTreg组分与pSS-IP或hcc相比也显著增加，同时与BAL中hcc相比，ⅲ组分增加。IPF患者来自胸腺的静息treg （rtreg）水平低于hcc患者，而来自胸腺的aTreg水平与hcc患者无显著差异。在IPF中，rtreg和aTregs均有增殖，aTregs的增殖能力强于rtreg。rTregs和aTregs均能显著抑制体外CD4+ T淋巴细胞的增殖。aTregs百分比与预测的一氧化碳扩散容量值呈负相关，与IPF的GAP指数呈正相关。我们的研究显示了IPF中Tregs亚群之间的不平衡。外周血aTregs比例升高与疾病严重程度呈负相关。
Regulatory T cells (Tregs) are crucial in maintaining immune tolerance and immune homeostasis, but their role in idiopathic pulmonary fibrosis (IPF) is unclear. This study was designed to explore the role of Tregs in IPF. Percentages of Tregs and their subpopulations in peripheral blood (PB) and bronchoalveolar lavage (BAL) samples were determined by flow cytometry in 29 patients with IPF, 19 patients with primary Sjögren’s syndrome-related interstitial pneumonia (pSS-IP), and 23 healthy controls (HCs). In peripheral blood, no difference was found in CD4+CD25+Foxp3+ Treg percentages among patients with IPF, pSS-IP, or HCs. However, activated Treg (aTreg) fractions among CD4+ T cells increased significantly in IPF compared with pSS-IP or HCs. Being consistent with the result from the PB, aTreg fractions among CD4+ T cells in IPF also increased significantly compared with pSS-IP or HCs, accompanied by increased fraction III compared with HCs in BAL. IPF patients had lower levels of resting Tregs (rTregs) from the thymus than did HCs, whereas aTreg levels originating from the thymus did not significantly differ from HCs. Both rTregs and aTregs proliferated in IPF, with aTregs being more proliferative than rTregs. Both rTregs and aTregs significantly inhibited proliferation of CD4+ T lymphocytes in vitro. The percentage of aTregs was correlated negatively with predicted diffusing capacity values for carbon monoxide and positively with GAP index in IPF. Our study showed the imbalance between subpopulations of Tregs in IPF. Increased aTregs proportion in the peripheral blood correlated inversely with disease severity.
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影响因子：
15.3
作者：
Kimmig, Sonja;Przybylski, Grzegorz K;Schmidt, Christian A;Laurisch, Katja;Mowes, Beate;Radbruch, Andreas;Thiel, Andreas
通讯作者：
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DOI：
10.1164/rccm.201103-0516oc
发表时间：
2011-12-01
影响因子：
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DOI：
10.1164/rccm.2009-040gl
发表时间：
2011-03-15
影响因子：
24.7
作者：
Raghu, Ganesh;Collard, Harold R.;Schuenemann, Holger J.
通讯作者：
Schuenemann, Holger J.
DOI：
10.1164/rccm.201202-0320st
发表时间：
2012-05-01
影响因子：
24.7
作者：
Meyer, Keith C.;Raghu, Ganesh;Wood, Brent
通讯作者：
Wood, Brent