Stress-Induced Changes in Trophic Factor Expression in the Rodent Urinary Bladder: Possible Links With Angiogenesis.
Stress-Induced Changes in Trophic Factor Expression in the Rodent Urinary Bladder: Possible Links With Angiogenesis.
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DOI：
10.5213/inj.2244118.059
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发表时间：
2022-12
影响因子：
2.3
通讯作者：
Birder LA
中科院分区：
文献类型：
--
作者：
de Rijk MM;Wolf-Johnston A;Kullmann AF;Maringer K;Sims-Lucas S;van Koeveringe GA;Rodríguez LV;Birder LA
大量证据支持慢性应激在膀胱功能障碍如间质性膀胱炎/膀胱疼痛综合征（IC/BPS）的发展、维持甚至增强中的作用。增加尿频和膀胱痛觉过敏已报道在啮齿动物暴露于慢性应激范式。本研究采用小鼠避水应激（WAS）模型，研究慢性应激对血管灌注和血管生成的影响。雌性Wistar-Kyoto大鼠连续10天暴露于WAS。膀胱颈部组织采用免疫印迹法检测血管内皮生长因子（VEGF）和神经生长因子前体（proNGF）。用荧光显微血管造影评估血管灌注，然后用缺氧探针检测组织缺氧区域。VEGF和proNGF在was大鼠膀胱颈黏膜中的表达明显高于对照组。血管灌注有增加的趋势，但与对照组相比无统计学差异。WAS大鼠灌注量增加1.6倍。此外，在was大鼠中观察到更丰富的血管，尤其是微血管。这些发现表明，慢性心理应激诱导因素可导致微血管形成增加，特别是在膀胱颈部周围，该区域包含大多数伤害性膀胱传入神经。这些发现可能表明血管生成与其他炎症因子之间存在联系，这些炎症因子有助于IC/BPS的结构改变和疼痛。
Substantive evidence supports a role of chronic stress in the development, maintenance, and even enhancement of functional bladder disorders such as interstitial cystitis/bladder pain syndrome (IC/BPS). Increased urinary frequency and bladder hyperalgesia have been reported in rodents exposed to a chronic stress paradigm. Here, we utilized a water avoidance stress (WAS) model in rodents to investigate the effect of chronic stress on vascular perfusion and angiogenesis. Female Wistar-Kyoto rats were exposed to WAS for 10 consecutive days. Bladder neck tissues were analyzed by western immunoblot for vascular endothelial growth factor (VEGF) and nerve growth factor precursor (proNGF). Vascular perfusion was assessed by fluorescent microangiography followed by Hypoxyprobe testing to identify regions of tissue hypoxia. The expression of VEGF and proNGF in the bladder neck mucosa was significantly higher in the WAS rats than in the controls. There was a trend toward increased vascular perfusion, but without a statistically significant difference from the control group. The WAS rats displayed a 1.6-fold increase in perfusion. Additionally, a greater abundance of vessels was observed in the WAS rats, most notably in the microvasculature. These findings show that chronic psychological stress induces factors that can lead to increased microvasculature formation, especially around the bladder neck, the region that contains most nociceptive bladder afferents. These findings may indicate a link between angiogenesis and other inflammatory factors that contribute to structural changes and pain in IC/BPS.
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影响因子：
4.5
作者：
Kiuchi, Hiroshi;Tsujimura, Akira;Okuyama, Akihiko
通讯作者：
Okuyama, Akihiko
DOI：
10.1152/ajprenal.2000.278.6.f905
发表时间：
2000-06-01
影响因子：
4.2
作者：
Kanellis, J;Fraser, S;Power, DA
通讯作者：
Power, DA
影响因子：
1.8
作者：
Kanter, Gregory;Komesu, Yuko M.;Rogers, Rebecca G.
通讯作者：
Rogers, Rebecca G.
影响因子：
4.5
作者：
Dagher A;Curatolo A;Sachdev M;Stephens AJ;Mullins C;Landis JR;van Bokhoven A;El-Hayek A;Froehlich JW;Briscoe AC;Roy R;Yang J;Pontari MA;Zurakowski D;Lee RS;Moses MA;MAPP Research Network
通讯作者：
MAPP Research Network
影响因子：
2.1
作者：
Lin, Hui-Yi;Park, Hyun Y.;Park, Jong Y.
通讯作者：
Park, Jong Y.