Transmural high energy phosphate distribution and response to alterations in workload in the normal canine myocardium as studied with spatially localized 31P NMR spectroscopy
Transmural high energy phosphate distribution and response to alterations in workload in the normal canine myocardium as studied with spatially localized 31P NMR spectroscopy
复制标题
使用空间局部 31P NMR 波谱研究正常犬心肌的透壁高能磷酸盐分布和对工作负荷变化的反应
DOI：
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发表时间：
1990
影响因子：
3.3
通讯作者：
K. Uğurbil
中科院分区：
文献类型：
--
作者：
P. Robitaille;H. Merkle;B. Lew;G. Path;K. Hendrich;P. Lindstrom;A. From;M. Garwood;R. Bache;K. Uğurbil
空间定位磷-31核磁共振（31 P NMR）光谱已应用于正常犬心肌的研究，以测量左心室壁高能磷酸盐的相对含量。使用FLAX-ISIS技术，在跨越左心室壁的五个体素中采集心室NMR数据。FLAX-ISIS方法在获取透壁研究的局部光谱和从局部光谱提供定量信息方面的有效性通过涉及体模、完整大鼠和犬体内心肌的研究进行了严格检查。结果表明：（1）该技术产生的空间分辨光谱相邻体素之间部分重叠，而每隔一个体素之间几乎没有重叠;（2）在犬心脏中，可以分别检测心外膜下、中壁和心内膜下的信号，而不会产生交叉污染;（3）可以定量体素内和体素之间的相对代谢物含量。在29只不同的动物上，在收缩早期或晚期，以及在心率峰值收缩压乘积（RPP）从6000 mmHg/min增加到35，000 mmHg/min的三种不同的工作负荷下，用心脏门控获得了心室31 P NMR谱。数据显示，在正常犬心肌中，心内膜下的磷酸肌酸（CP）含量和CP/ATP比值明显低于心外膜下。ATP水平是恒定的。无论是CP含量和CP/ATP比测量每个体素保持不变的心动周期的相位或工作量的104倍的增加。每个体素计算的游离ADP水平表明，ADP是相对较高的subendocardium比subepicardium，并在所有透壁层高于其表观Km的氧化磷酸化。在该领域中，ADP含量随工作负荷和MVO 2的变化是不可预期的，也未观察到。出版社：Academic Press，Inc.
Spatially localized phosphorus‐31 nuclear magnetic resonance (31P NMR) spectroscopy has been applied to the study of the normal canine myocardium to measure the relative content of high energy phosphates across the left ventricular wall. Transmural NMR data were acquired in five voxels spanning the wall of the left ventricle using the FLAX‐ISIS technique. The validity of the FLAX‐ISIS approach in acquiring localized spectra for transmural studies and in providing quantitative information from the localized spectra was examined rigorously by studies involving phantoms, intact rats, and the canine myocardium in vivo. The results indicated that (1) this technique yields spatially resolved spectra with partial overlap between adjacent voxels and virtually no overlap between every other voxel; (2) in the canine heart, signals from subepicardium, midwall, and sub‐endocardium can be detected separately without cross contamination; and (3) relative metabolite contents within a voxel and among voxels can be quantitated. Transmural 31P NMR spectra were acquired with cardiac gating on 29 separate animals either at early systole or late diastole, and at three different workloads with the heart rate peak systolic pressure product (RPP) increasing from 6000 mmHg/min to 35,000 mmHg/min. The data revealed that in the normal canine myocardium, the creatine phosphate (CP) content and the CP/ATP ratio was significantly lower in the subendocardium than in the subepicardium. ATP levels were transmurally constant. Both the CP content and the CP/ATP ratio measured for each voxel remained unaltered in relation to either the phase of the cardiac cycle or ∼ fourfold increase in workload. Free ADP levels calculated for each voxel showed that ADP was relatively higher in the subendocardium than the subepicardium, and in all transmural layers was higher than its apparent Km for oxidative phosphorylation. In this domain changes in ADP content with workload and MVO2 are not expected and were not observed. © 1990 Academic Press, Inc.
通过仔细控制再灌注条件和再灌注液成分，可在局部缺血六小时后立即恢复功能。
DOI：
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发表时间：
1986
期刊：
The Journal of thoracic and cardiovascular surgery
影响因子：
--
作者：
Allen,BS;Okamoto,F;Buckberg,GD;Bugyi,H;Young,H;Leaf,J;Beyersdorf,F;Sjostrand,F;MaloneyJr,JV
通讯作者：
MaloneyJr,JV
局部缺血后手术再灌注与药物再灌注的优越性。
DOI：
--
发表时间：
1986
期刊：
The Journal of thoracic and cardiovascular surgery
影响因子：
--
作者：
Vinten-Johansen,J;Buckberg,GD;Okamoto,F;Rosenkranz,ER;Bugyi,H;Leaf,J
通讯作者：
Leaf,J
影响因子：
56.9
作者：
BALABAN, RS;KANTOR, HL;BRIGGS, RW
通讯作者：
BRIGGS, RW