The relative composition of the inflammatory infiltrate as an additional tool for synovial tissue classification.
The relative composition of the inflammatory infiltrate as an additional tool for synovial tissue classification.
复制标题
DOI：
10.1371/journal.pone.0072494
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Pessler F
中科院分区：
文献类型：
--
作者：
Della Beffa C;Slansky E;Pommerenke C;Klawonn F;Li J;Dai L;Schumacher HR Jr;Pessler F
传统上，表达某种标记物的细胞绝对数的差异(例如，阳性染色细胞/mm2)一直被用于免疫组织学滑膜组织分类。我们已经开始评估炎性浸润物的相对成分，即炎性细胞类型在炎性浸润物中的百分比，作为一种替代分类工具，可能会改善组织诊断，临床试验中的亚型划分，以及对炎性和非炎症性关节病发病机制的理解。滑膜组织标本(正常滑膜15例，骨性关节病6例，骨性关节炎26例，早期未分化关节炎10例，类风湿关节炎26例，慢性化脓性关节炎11例)CD15、CD68、CD3、CD20、CD38染色。在浅层内膜下测定表达特定标记物的细胞密度。采用二分类和多分类接收器工作特征(ROC)分析和朴素贝叶斯分类器比较(1)表达某一标记物的细胞的绝对密度(绝对法)和(2)这些细胞在炎性细胞群体中的百分比(相对法)，以区分六种组织类别。正常滑膜和骨科关节病的炎症细胞几乎全部由CD68+和CD3+细胞组成。显著的CD20+和CD38+细胞出现在骨关节炎样本的子集中，并且在早期、类风湿性和慢性化脓性关节炎中进一步增加。ROC分析和朴素贝叶斯分类器将绝对方法的总体判别能力排在相对方法之上。当样本也按炎性细胞总数/mm2分层时，相对方法变得略有优势。这项以各种关节疾病为特征的探索性调查显示，如果样本也根据炎症强度进行分层，那么测量炎症细胞类型的相对比例可能有助于滑膜组织的分类。
Traditionally, differences in absolute numbers of cells expressing a certain marker (e.g., positive staining cells per mm2) have been used in immunohistological synovial tissue classification. We have begun to evaluate the relative composition of the inflammatory infiltrates, i.e. percentages of inflammatory cell types in inflammatory infiltrates, as an alternate classification tool that may potentially improve tissue diagnostics, subgrouping in clinical trials, and understanding of pathogenesis of inflammatory and noninflammatory arthropathies. Synovial tissue specimens (normal synovium, n=15; orthopedic arthropathies, n=6; osteoarthritis, n=26; early undifferentiated arthritis, n=10; rheumatoid arthritis, n=26; chronic septic arthritis, n=11) were stained for CD15, CD68, CD3, CD20, and CD38. Densities of cells expressing a given marker were determined in the superficial subintima. Binary and multicategory receiver operating characteristic (ROC) analysis and naïve Bayes classifier were used to compare the abilities of (1) the absolute densities of cells expressing a given marker (absolute method) with (2) the percentages of these cells in the inflammatory cell population (relative method) to differentiate among the six tissue classes. The inflammatory infiltrates in normal synovium and the orthopedic arthropathies consisted almost exclusively of CD68+ and CD3+ cells. Notable fractions of CD20+ and CD38+ cells appeared in a subset of osteoarthritis samples, and increased further in early, rheumatoid and chronic septic arthritis. ROC analyses and naïve Bayes classifier ranked the absolute method above the relative method in terms of overall discriminatory ability. The relative method became slightly superior when the samples were also stratified according to the total number of inflammatory cells/mm2. This exploratory investigation featuring a variety of joint disorders revealed that measuring the relative proportions of inflammatory cell types may aid in synovial tissue classification if the samples are also stratified according to the intensity of inflammation.
登录
查看更多内容
影响因子：
2.8
作者：
Krenn, V;Morawietz, L;König, A
通讯作者：
König, A
DOI：
10.1002/art.23006
发表时间：
2007-10-15
期刊：
ARTHRITIS & RHEUMATISM-ARTHRITIS CARE & RESEARCH
影响因子：
--
作者：
Diaz-Torne, C.;Schumacher, H. R.;Pessler, F.
通讯作者：
Pessler, F.
影响因子：
27.4
作者：
Pessler, F.;Ogdie, A.;Schumacher, H. R.
通讯作者：
Schumacher, H. R.
DOI：
10.1002/art.23915
发表时间：
2008-08-15
期刊：
ARTHRITIS & RHEUMATISM-ARTHRITIS CARE & RESEARCH
影响因子：
--
作者：
Pessler, F.;Dai, L.;Schumacher, H. R.
通讯作者：
Schumacher, H. R.
影响因子：
27.4
作者：
Pessler, F.;Dai, L.;Schumacher, H. R.
通讯作者：
Schumacher, H. R.