Electrophysiological and morphological properties of neurons in layer 5 of the rat postrhinal cortex.
Electrophysiological and morphological properties of neurons in layer 5 of the rat postrhinal cortex.
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DOI：
10.1002/hipo.22026
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发表时间：
2012-09
期刊：
影响因子：
3.5
通讯作者：
Burwell, Rebecca D.
中科院分区：
文献类型：
--
作者：
Sills, Joseph B.;Connors, Barry W.;Burwell, Rebecca D.
大鼠的嗅后皮质（POR）与灵长类动物的海马旁皮质基于连接和其他标准是同源的。后嗅皮层提供了主要的视觉和视觉空间输入到海马结构，直接到CA 1和间接通过连接与内侧内嗅皮层。尽管对后嗅皮层的皮层和海马的联系有了很好的描述，但对后嗅神经元的生理学还没有研究。本文采用离体切片法，观察了14-16日龄大鼠嗅后皮质第5层神经元的电生理和形态学特征。根据神经元的电生理特性和与新皮层其他区域神经元的相似性，将神经元主观地分为规则尖峰（RS）、快速尖峰（FS）或低阈值尖峰（LTS）。用生物胞素染色的细胞包括锥体细胞和具有双簇状或多极树突模式的中间神经元。仅使用生理数据的相似性分析产生了三个聚类，对应于FS，LTS和RS类。对应于FS类的群集完全由多极非锥体细胞组成，而对应于RS类的群集完全由锥体细胞组成。第三簇，对应于LTS类，是异质的，包括多极和双簇树突状乔木以及一个锥体细胞。我们没有观察到任何内在的爆裂锥体细胞，这是类似于内嗅皮层，但不同于嗅周皮层。我们的结论是，POR包括至少两个主要类别的新皮层抑制性中间神经元，但有一个功能受限的队列的锥体细胞。
The postrhinal cortex (POR) of the rat is homologous to the parahippocampal cortex of the primate based on connections and other criteria. Postrhinal cortex provides the major visual and visuospatial input to the hippocampal formation, both directly to CA1 and indirectly through connections with the medial entorhinal cortex. Although the cortical and hippocampal connections of the postrhinal cortex are well described, the physiology of postrhinal neurons has not been studied. Here we examined the electrical and morphological characteristics of layer 5 neurons from postrhinal cortex of 14-16 day-old rats using an in vitro slice preparation. Neurons were subjectively classified as regular-spiking (RS), fast-spiking (FS) or low threshold-spiking (LTS) based on their electrophysiological properties and similarities with neurons in other regions of neocortex. Cells stained with biocytin included pyramidal cells and interneurons with bitufted or multipolar dendritic patterns. Similarity analysis using only physiological data yielded three clusters that corresponded to FS, LTS, and RS classes. The cluster corresponding to the FS class was composed entirely of multipolar nonpyramidal cells, and the cluster corresponding to the RS class was composed entirely of pyramidal cells. The third cluster, corresponding to the LTS class, was heterogeneous and included both multipolar and bitufted dendritic arbors as well as one pyramidal cell. We did not observe any intrinsically bursting pyramidal cells, which is similar to entorhinal cortex but unlike perirhinal cortex. We conclude that POR includes at least two major classes of neocortical inhibitory interneurons, but has a functionally restricted cohort of pyramidal cells.
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影响因子：
2.5
作者：
KASPER, EM;LARKMAN, AU;BLAKEMORE, C
通讯作者：
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Burwell, Rebecca D.
影响因子：
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作者：
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BLAKEMORE, C
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作者：
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