Histopathologic and immunochemical features of lattice corneal dystrophy type III.
Histopathologic and immunochemical features of lattice corneal dystrophy type III.
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III 型格子状角膜营养不良的组织病理学和免疫化学特征。
DOI：
10.1016/0002-9394(87)90412-0
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发表时间：
1987
影响因子：
4.2
通讯作者：
Klintworth,GK
中科院分区：
文献类型：
--
作者：
Hida,T;Proia,AD;Kigasawa,K;Sanfilippo,FP;BurchetteJr,JL;Akiya,S;Klintworth,GK
我们用光镜和电子显微镜检查了5例新型晶格型角膜营养不良(指定为III型晶格型角膜营养不良)患者的7个角膜。大量淀粉样蛋白沉积散布在角膜基质中，其中一些比I型或II型晶格状角膜营养不良中通常观察到的要大得多；这些沉积主要位于上皮和内皮之间。图像分析显示，与年龄匹配的I型晶格状角膜营养不良患者相比，大量基质淀粉样沉积的横截面积明显增大。所有患者在鲍曼层下的浅基质中都有一条不连续的淀粉样带(宽15~25μm)，通常只有一到两个小的断裂。Descemet膜和内皮细胞正常。间质沉积物由直径10 nm的淀粉样蛋白典型的纤维组成，组织切片经稀高锰酸钾处理后刚果红染色阳性。对福尔马林固定的石蜡包埋组织的免疫组织化学研究表明，只有一些沉积物与淀粉样蛋白AA的抗体反应较弱。这些沉淀物与AP蛋白抗体呈阳性反应，与kappa和lambda免疫球蛋白轻链抗体呈阴性反应。
We examined seven corneas from five patients with a new form of lattice corneal dystrophy (designated lattice corneal dystrophy type III) by light and electron microscopy. Numerous amyloid deposits were scattered throughout the corneal stroma, some of which were much larger than those usually observed in either lattice corneal dystrophy type I or II; these were located predominantly midway between the epithelium and the endothelium. Image analysis disclosed that the cross-sectional size of the large stromal amyloid deposits was significantly greater than those in age-matched patients with lattice corneal dystrophy type I. All patients had a discontinuous band of amyloid (15 to 25 μm wide) in the superficial stroma beneath Bowman's layer, which usually had only one or two small disruptions. Descemet's membrane and the endothelium were normal. The stromal deposits, which were composed of 10-nm diameter fibrils typical of amyloid, stained positively with Congo red after the histologic sections were pretreated with dilute potassium permanganate. Immunohistochemical studies on formalin-fixed, paraffin-embedded tissue indicated that only some deposits reacted weakly with antibodies to amyloid protein AA. The deposits stained positively with antibodies to protein AP and negatively with antibodies to kappa and lambda immunoglobulin light chains.
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淀粉样蛋白的生物学意义 *
影响因子：
5.2
作者：
P. Gorevic;Alice B. Cleveland;E. Franklin
通讯作者：
E. Franklin
眼部疾病病理学
DOI：
--
发表时间：
1983
期刊：
影响因子：
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作者：
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F. Blodi
遗传性淀粉样变性伴格子状角膜营养不良和继发性淀粉样变性中淀粉样蛋白的部分特征。
DOI：
--
发表时间：
1978
期刊：
Medical biology
影响因子：
--
作者：
J. Meretoja;T. Hollmén;T. Meretoja;R. Penttinen
通讯作者：
R. Penttinen
DOI：
10.1111/j.1749-6632.1982.tb22144.x
发表时间：
1982-01-01
影响因子：
5.2
作者：
PEPYS, MB;BALTZ, ML;FEINSTEIN, A
通讯作者：
FEINSTEIN, A
福尔马林固定石蜡切片中淀粉样蛋白的免疫细胞化学鉴定。
DOI：
10.1007/bf00517130
发表时间：
1981
期刊：
Histochemistry
影响因子：
--
作者：
Shirahama,T;Skinner,M;Cohen,AS
通讯作者：
Cohen,AS