In vivo cartilage formation from growth factor modulated articular chondrocytes.
In vivo cartilage formation from growth factor modulated articular chondrocytes.
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体内软骨由生长因子调节的关节软骨细胞形成。
DOI：
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发表时间：
1998
影响因子：
4.2
通讯作者：
W. Horton
中科院分区：
文献类型：
--
作者：
D. Bradham;W. Horton
由于随着年龄的增长，干细胞和软骨细胞的丢失，以及老化软骨中软骨细胞的体外增殖缓慢，近期的自体修复老年患者软骨缺损的手术可能很困难。本研究通过生长因子调节关节软骨细胞的表型，并在培养中刺激这些细胞的增殖，获得了次生软骨祖细胞。从成熟新西兰大白兔关节软骨中分离的软骨细胞暴露于转化生长因子β和碱性成纤维细胞生长因子的联合处理下。这些细胞停止产生胶原II（软骨细胞表型的标志），细胞数量增加了136倍。接下来，将细胞置于高密度培养中，在体外重新表达软骨细胞表型，并在体内实验中形成透明软骨。从老年人关节软骨中获得的原代软骨细胞可以在体外以类似的方式进行操作。当在体内和组织生物反应器中检测时，这些人次生软骨祖细胞仅形成软骨组织。这种方法对于老年骨关节炎患者退行性关节软骨的自体修复可能是必要的。
Recent procedures for autologous repair of cartilage defects may be difficult in elderly patients because of the loss of stem cells and chondrocytes that occurs with age and the slow in vitro proliferation of chondrocytes from aged cartilage. In this study secondary chondroprogenitor cells were obtained by modulating the phenotype of articular chondrocytes with growth factors and stimulating the proliferation of these cells in culture. Chondrocytes isolated from the articular cartilage of mature New Zealand White rabbits were exposed to a combination of transforming growth factor beta and basic fibroblast growth factor treatment. These cells ceased the production of Collagen II (a marker for the chondrocyte phenotype) and underwent a 136-fold increase in cell number. Next, the cells were placed in high density culture and reexpressed the chondrocyte phenotype in vitro and formed hyaline cartilage in an in vivo assay. Primary chondrocytes obtained from articular cartilage of elderly humans could be manipulated in a similar fashion in vitro. These human secondary chondroprogenitor cells formed only cartilage tissue when assayed in vivo and in tissue bioreactors. This approach may be essential for autologous repair of degenerated articular cartilage in elderly patients with osteoarthritis.
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通过将白细胞介素 1 受体拮抗剂基因转移至滑膜来抑制关节内对白细胞介素 1 的反应。
影响因子：
5.1
作者：
Hung,GL;Galea-Lauri,J;Mueller,GM;Georgescu,HI;Larkin,LA;Suchanek,MK;Tindal,MH;Robbins,PD;Evans,CH
通讯作者：
Evans,CH
影响因子：
2.7
作者：
J. Seed;B. Olwin;S. Hauschka
通讯作者：
J. Seed;B. Olwin;S. Hauschka
使用腺病毒载体转导人 IL-1 受体拮抗剂 cDNA 来抑制滑膜细胞中白细胞介素 1 诱导的作用。
DOI：
10.1089/hum.1995.6.3-307
发表时间：
1995
期刊：
Human gene therapy.
影响因子：
--
作者：
Roessler,BJ;Hartman,JW;Vallance,DK;Latta,JM;Janich,SL;Davidson,BL
通讯作者：
Davidson,BL
DOI：
10.1073/pnas.90.21.10404
发表时间：
1993-11-01
影响因子：
11.1
作者：
COHEN, IR;GRASSEL, S;IOZZO, RV
通讯作者：
IOZZO, RV