Secreted metalloproteinases in testicular cell culture.
Secreted metalloproteinases in testicular cell culture.
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睾丸细胞培养物中分泌的金属蛋白酶。
DOI：
10.1095/biolreprod43.6.946
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发表时间：
1990
影响因子：
3.6
通讯作者：
Byers,SW
中科院分区：
文献类型：
--
作者：
Sang,QX;Dym,M;Byers,SW
众所周知，培养的Sertoli细胞分泌纤溶酶原激活剂（Lacroix等人，Mol Cell Endocrinol 1977; 9：227-236; Hettle等人，Biol Reprod 1986; 34：895-904）。我们现在发现培养的睾丸细胞也分泌明胶金属蛋白酶。浓缩培养基蛋白的明胶酶谱分析揭示，支持细胞分泌的明胶酶的185 kDa，110 kDa，83 kDa，76 kDa，和72 kDa的纤溶酶原激活剂（PA）。明胶酶185 kDa由FSH诱导。支持（上皮）/管周（间充质）细胞共培养的培养基中含有支持细胞明胶酶和一种FSH刺激的50 kDa明胶酶，表明明胶酶50 kDa受FSH和细胞-细胞相互作用的调节。在FSH存在下生长的细胞的共培养基中也存在50-kDa纤连蛋白溶解活性。酪蛋白酶谱显示一个突出的30 kDa的蛋白酶只在媒体从共培养。管周细胞分泌尿激酶型纤溶酶原激活剂（u-PA），并在86 kDa和74 kDa处表现出轻微的降解活性。明胶酶在pH 7.3-8.5范围内最具活性，并且完全或部分地被金属离子螯合剂抑制，表明它们是金属蛋白酶。我们的数据表明，培养的睾丸细胞分泌几种明胶酶，除了PA，FSH和共培养条件下调节这些分泌的蛋白酶。我们认为，这些蛋白酶的高度调节分泌可能是在睾丸发育和精子发生的生理重要性。
It is well known that cultured Sertoli cells secrete plasminogen activators (Lacroix et al., Mol Cell Endocrinol 1977; 9:227–236; Hettle et al., Biol Reprod 1986; 34:895–904). We now show that testicular cells in culture also secrete gelatinolytic metalloproteinases. Gelatin zymographic analysis of concentrated culture medium proteins reveals that Sertoli cells secrete gelatinases of 185 kDa, 110 kDa, 83 kDa, 76 kDa, and 72 kDa in addition to plasminogen activators (PAs). Gelatinase 185 kDa is induced by FSH. Media from Sertoli (epithelial)/peritubular (mesenchymal) cell cocultures contain the Sertoli cell gelatinases and one FSH-stimulated gelatinase of 50 kDa, indicating that gelatinase 50 kDa is regulated by both FSH and cell-cell interactions. A 50-kDa fibronectinolytic activity is also present in the coculture medium from cells grown in the presence of FSH. Casein zymography demonstrates a prominent 30-kDa protease only in media from cocultures. Peritubular cells secrete urokinase-type plasminogen activator (u-PA) and exhibit slight degrading activity at 86 kDa and 74 kDa. The gelatinases are most active in the pH range 7.3–8.5 and are completely or partially inhibited by metal ion chelators indicating that they are metalloproteinases. Our data demonstrate that testicular cells in culture secrete several gelatinases in addition to PAs, and that FSH and coculture conditions regulate some of these secreted proteases. We suggest that the highly regulated secretion of these proteases may well be of physiological importance during testicular development and spermatogenesis.
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大鼠睾丸细胞培养物中 IV 型胶原酶的鉴定：管周支持细胞相互作用的影响。
影响因子：
3.6
作者：
Sang,QX;Stetler-Stevenson,WG;Liotta,LA;Byers,SW
通讯作者：
Byers,SW
DOI：
10.1073/pnas.77.9.5331
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
JOHNSONWINT, B
通讯作者：
JOHNSONWINT, B
共培养中支持细胞和睾丸管周细胞的形态发生重组和基底膜形成：通过环 AMP 衍生物和阻断直接细胞接触抑制形态发生级联。
影响因子：
2.7
作者：
P. S. Tung;Irving B. Fritz
通讯作者：
Irving B. Fritz
来自人类类风湿滑膜成纤维细胞的金属蛋白酶，可消化结缔组织基质成分。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Okada,Y;Nagase,H;HarrisJr,ED
通讯作者：
HarrisJr,ED
DOI：
10.1111/j.1749-6632.1987.tb25006.x
发表时间：
1987-12
影响因子：
5.2
作者：
M. Skinner
通讯作者：
M. Skinner