Suppression of potyvirus infection by coexpressed closterovirus protein.
Suppression of potyvirus infection by coexpressed closterovirus protein.
复制标题
通过共表达的长线病毒蛋白抑制马铃薯Y病毒感染。
DOI：
10.1006/viro.1997.8660
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Peremyslov,VV
中科院分区：
文献类型：
--
作者：
Dolja,VV;Hong,J;Keller,KE;Martin,RR;Peremyslov,VV
基于烟草蚀刻病毒(TEV)的表达载体被用来插入来自无关甜菜黄化病毒(BYV)的几个ORF。表达BYV衣壳蛋白、20 kDa蛋白或HSP70同源蛋白的混合TEV变体系统感染烟草并稳定保留BYV序列。相反，插入编码BYV前导蛋白的ORF(L-Pro)会严重损害重组TEV的系统转运和积聚。该病毒的后代经历了不同程度的缺失，影响了L-Pro序列，缓解了病毒传播的缺陷。几个自发和工程突变体的模型实验表明，黄矮病病毒L-Pro的中心结构域是造成杂交病毒积累缺陷的原因，而全尺寸L-Pro是最大限度地削弱系统转运所必需的。值得注意的是，黄矮病病毒L-Pro的表达并没有减弱杂交烟草病毒对烟草的系统侵染。在INN中没有发现重组RNA的复制或包装方面的主要缺陷。烟草原生质体。这些结果表明，黄瓜花叶病毒L-Pro以宿主依赖的方式特异性地干扰TEV的系统转运和积累，提示闭锁病毒L-Pro作为马铃薯Y病毒感染的抑制因子具有潜在的应用前景。此外，TEV辅助组分蛋白酶的107个氨基酸残基的N末端部分被证明不是系统感染所必需的。
A tobacco etch virus (TEV)-based expression vector has been used for insertion of several ORFs derived from the unrelated beet yellows virus (BYV). Hybrid TEV variants expressing the BYV capsid protein, 20-kDa protein, or HSP70 homolog systemically infectedNicotiana tabacumand stably retained BYV sequences. In contrast, insertion of the ORF encoding BYV leader proteinase (L-Pro) resulted in severely impaired systemic transport and accumulation of recombinant TEV. Progeny of this virus underwent various deletions affecting the L-Pro sequence and mitigating the defects in virus spread. Model experiments involving several spontaneous and engineered mutants indicated that the central domain of BYV L-Pro was responsible for the defect in hybrid virus accumulation, whereas full-size L-Pro was required for maximal debilitation of systemic transport. Strikingly, BYV L-Pro expression did not debilitate systemic infection of hybrid TEV inNicotiana benthamianaplants. No major defects in replication or encapsidation of recombinant RNA were revealed inN. tabacumprotoplasts. These results indicated that BYV L-Pro specifically interfered with TEV systemic transport and accumulation in a host-dependent manner and suggested a potential utility of closterovirus L-Pro as an inhibitor of potyvirus infection. In addition, it was demonstrated that the 107-amino-acid-residues-long N-terminal part of the TEV helper component proteinase is not essential for systemic infection.
登录
查看更多内容
影响因子：
3.7
作者：
Kasschau, KD;Cronin, S;Carrington, JC
通讯作者：
Carrington, JC
影响因子：
5.6
作者：
AGRANOVSKY, AA;BOYKO, VP;DOLJA, VV
通讯作者：
DOLJA, VV
影响因子：
3.7
作者：
Karasev, AV;Nikolaeva, OV;Dawson, WO
通讯作者：
Dawson, WO
DOI：
10.1073/pnas.92.7.2470
发表时间：
1995-03-28
影响因子：
11.1
作者：
AGRANOVSKY, AA;LESEMANN, DE;ATABEKOV, JG
通讯作者：
ATABEKOV, JG
柑橘 tristeza 病毒 RNA 积累的动力学。
影响因子：
3.7
作者：
J. Navas;M. R. Albiach;S. Gowda;M. Hilf;S. Garnsey;W. O. Dawson
通讯作者：
W. O. Dawson