Effect of Oral Administration of CpG ODN-OVA on WBB 6 F 1W Wv Mice
Effect of Oral Administration of CpG ODN-OVA on WBB 6 F 1W Wv Mice
复制标题
口服 CpG ODN-OVA 对 WBB 6 F 1W Wv 小鼠的影响
DOI：
--
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
J. Sawada
中科院分区：
文献类型：
--
作者：
R. Teshima;H. Okunuki;Yuji Sato;H. Akiyama;T. Maitani;J. Sawada
背景：我们已报道WBB6F1-W WV(W WV)小鼠口服卵清蛋白(OVA)后产生极高的抗原特异性IgG1抗体。小鼠腹腔注射后，诱发活动性全身过敏反应(ASA)。给予OVA，Th2占优势的辅助性T细胞被激活。本研究探讨了卵清蛋白(OVA)的CpG寡核苷酸(ODN)偶联对WV小鼠口服免疫的影响。方法：用0.1 mg OVA或CpG ODN-OVA每日灌胃致敏W WV小鼠，连续4周，测定血清中OVA特异性IgG1、IgE和IgG2a抗体效价。AsA由腹腔注射诱导。注射卵清蛋白，监测体温变化。同时检测抗原刺激的脾细胞体外产生Th1和Th2类细胞因子的情况。结果：CpG ODN-OVA致敏的WWV小鼠的抗原特异性IgG1抗体滴度低于OVA致敏组，而IgG2a滴度高于OVA致敏组。AsA不是由ip诱导的。OVA挑战赛。CpGODN-OVAS致敏小鼠脾细胞产生Th1型细胞因子(干扰素-γ)明显增加，但脾细胞培养上清液中Th2型细胞因子(IL-4)水平较低。结论：口服CpG ODN-OVA结合物可显著诱导抗原特异性Th1反应，降低Th2反应(过敏反应)。这些发现表明，CpG ODN-抗原结合物可作为口服疫苗使用。
Background: We have already reported that antigen-specific IgG1 antibody production in WBB6F1-W Wv (W Wv) mice after oral administration of ovalbumin (OVA) was extremely high. Active systemic anaphylaxis (ASA) was induced in these mice after intraperitoneal (i.p.) administration of OVA, and Th2-dominant helper Tcell activation occurred. In this study, we examined the effect of CpG oligodeoxynucleotide (ODN) conjugation of OVA on oral immunization of W Wv mice. Methods: W Wv mice were sensitized by administration of 0.1 mg OVA or CpG ODN-OVA by gavage every day for 4 weeks, and the serum titers of OVA-specific IgG1, IgE, and IgG2a antibody were determined. ASA was induced by i.p. injection of OVA, and the changes in body temperature were monitored. In vitro production of Th1and Th2type cytokines by splenocytes re-stimulated with antigen was also measured. Results: The antigen-specific IgG1 antibody titer in the CpG ODN-OVA-sensitized W Wv mice was lower than in the OVA-sensitized group, but the IgG2a titer was higher. ASA was not induced by i.p. OVA challenge. There were significant increases in the production of Th1-type cytokine (IFN-γ) by splenocytes in the CpG ODN-OVAsensitized mice, but the Th2-type cytokine (IL-4) level in the splenocyte culture medium was lower. Conclusions: These results indicated that oral administration of CpG ODN-OVA conjugate significantly induced antigen-specific Th1 responses and reduced Th2 responses (allergic reactions) on re-stimulation. These findings suggest that CpG ODN-antigen conjugate may be useful as an oral vaccine.
影响因子：
10.5
作者：
NOCKA, K;MAJUMDER, S;BESMER, P
通讯作者：
BESMER, P
优化的缀合比例导致过敏原免疫刺激性寡脱氧核苷酸缀合物保留免疫原性和最小的过敏原性。
DOI：
10.1067/mai.2002.126660
发表时间：
2002
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Horner,AnthonyA;Takabayashi,Kenji;Beck,Lucinda;Sharma,Bhavya;Zubeldia,Jose;Baird,Stephen;Tuck,Stephan;Libet,Lev;Spiegelberg,HansL;Liu,Fu-Tong;Raz,Eyal
通讯作者：
Raz,Eyal
影响因子：
14.2
作者：
Tighe, H;Takabayashi, K;Raz, E
通讯作者：
Raz, E