Ovariectomy increases L-type Ca(2+) channel activity in porcine coronary smooth muscle.
Ovariectomy increases L-type Ca(2+) channel activity in porcine coronary smooth muscle.
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卵巢切除术增加猪冠状动脉平滑肌中L型Ca(2)通道的活性。
DOI：
10.1097/gme.0000000000000087
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Bowles,DouglasK
中科院分区：
文献类型：
--
作者：
Tharp,DarlaL;Ivey,JanR;Shaw,RebeccaL;Bowles,DouglasK
目的性激素状态已被证明在离子通道活性调节中起作用。我们先前证实雄性猪冠状动脉平滑肌细胞(SMC)的L型钙通道电流(ICa)高于雌性猪。在公猪中，内源性睾酮通过促进造孔α1亚单位Cav1.2的表达而增加SMC中的ICa。相反，性激素在母猪身上的作用以前还没有被研究过。因此，本研究旨在探讨卵巢摘除对雌性尤卡坦小型猪L型钙通道活性和表达的影响。方法从种猪那里获得性成熟的雌性猪，并将其作为正常雌性猪(n=5)和去卵巢的雌性猪(n=6)。相应地，去卵巢组(−9.5[0.6]pA/pF)较假手术组(−4.5[0.3]pA/pF)的平均(扫描电子显微镜)ICa升高，而L型钙通道α1亚单位(Cav1.2；α1c)信使核糖核酸和蛋白表达无明显变化。
ObjectiveSex hormone status has been demonstrated to play a role in the regulation of ion channel activity. We previously demonstrated increased L-type Ca 2+ channel current (I Ca) in the coronary smooth muscle cells (SMCs) of male swine compared with female swine. In male swine, endogenous testosterone increases I Ca in SMCs by enhanced expression of the pore-forming α1 subunit Ca v 1.2. Conversely, the role of sex hormones in female swine has not previously been investigated. Therefore, the purpose of the current study was to determine the effect of ovariectomy (OVX) on L-type Ca 2+ channel activity and expression in female Yucatan miniature swine.MethodsSexually mature female swine were obtained from a breeder and either left intact (intact female [IF]; n= 5) or ovariectomized (n= 6).ResultsSensitivity to depolarization-induced contractions was increased by OVX. Accordingly, mean (SEM) I Ca was enhanced in the OVX group (− 9.5 [0.6] pA/pF) compared with the IF group (− 4.5 [0.3] pA/pF), although L-type Ca 2+ channel α1 subunit (Ca v 1.2; α1c) messenger RNA (mRNA) and protein expressions were unchanged.
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DOI：
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发表时间：
1993
期刊：
Journal of Physiology
影响因子：
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DOI：
10.1016/s0022-2828(87)80388-7
发表时间：
1987-08-01
影响因子：
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作者：
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DOI：
--
发表时间：
1990
期刊：
Reviews of Physiology, Biochemistry and Pharmacology
影响因子：
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