A novel Botrytis species is associated with a newly emergent foliar disease in cultivated Hemerocallis.
A novel Botrytis species is associated with a newly emergent foliar disease in cultivated Hemerocallis.
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DOI：
10.1371/journal.pone.0089272
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Dewey FM
中科院分区：
文献类型：
--
作者：
Grant-Downton RT;Terhem RB;Kapralov MV;Mehdi S;Rodriguez-Enriquez MJ;Gurr SJ;van Kan JA;Dewey FM
对表现出一种新出现的被称为“春病”的叶部病害症状的栽培黄花菜（大花萱草属杂交种）的叶组织样品进行了相关真菌的调查。这种疾病的病因尚不清楚。我们反复分离到一种真菌，根据免疫学试验证明它是葡萄孢属的成员。这些菌株的DNA序列分析，使用几个不同的phyogenetically信息基因，表明它们代表一个新的葡萄孢属物种，最密切相关的B。椭圆叶百合（lily blight，fire blight）是栽培百合的主要病原菌。通过对无性产孢培养物的形态学分析证实了分离株的区别。离体条件下对萱草组织的致病性试验表明，该新种能引起组织病变和快速坏死。根据这些数据，我们推断这个新物种，这里描述为B。deweyae，可能是一个重要的贡献者发展的“春季疾病”的症状。发病机制可能是促进发展和环境因素，有利于攻击这种necrotrophic病原体。这种疾病的出现被认为是由栽培杂交种的育种相关变化，特别是遗传多样性的侵蚀引发的。我们的调查证实，紧急的植物病害是重要的，值得密切监测，特别是在密集的近交植物。
Foliar tissue samples of cultivated daylilies (Hemerocallis hybrids) showing the symptoms of a newly emergent foliar disease known as ‘spring sickness’ were investigated for associated fungi. The cause(s) of this disease remain obscure. We isolated repeatedly a fungal species which proved to be member of the genus Botrytis, based on immunological tests. DNA sequence analysis of these isolates, using several different phyogenetically informative genes, indicated that they represent a new Botrytis species, most closely related to B. elliptica (lily blight, fire blight) which is a major pathogen of cultivated Lilium. The distinction of the isolates was confirmed by morphological analysis of asexual sporulating cultures. Pathogenicity tests on Hemerocallis tissues in vitro demonstrated that this new species was able to induce lesions and rapid tissue necrosis. Based on this data, we infer that this new species, described here as B. deweyae, is likely to be an important contributor to the development of ‘spring sickness’ symptoms. Pathogenesis may be promoted by developmental and environmental factors that favour assault by this necrotrophic pathogen. The emergence of this disease is suggested to have been triggered by breeding-related changes in cultivated hybrids, particularly the erosion of genetic diversity. Our investigation confirms that emergent plant diseases are important and deserve close monitoring, especially in intensively in-bred plants.
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影响因子：
1.2
作者：
Furukawa, Toshiko;Ushiyama, Kinji;Kishi, Kunihei
通讯作者：
Kishi, Kunihei
影响因子：
1.5
作者：
Chiou, AL;Wu, WS
通讯作者：
Wu, WS
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
DOI：
10.1080/00288233.1978.10427471
发表时间：
1978-01-01
影响因子：
1.8
作者：
LAUNDON, GF
通讯作者：
LAUNDON, GF
影响因子：
1.9
作者：
Mueller, DS;Williams-Woodward, JL;Buck, JW
通讯作者：
Buck, JW