A theoretical framework for the analysis of optical anisotropy in birefringent biological tissues with polarization-sensitive optical coherence tomography

A theoretical framework for the analysis of optical anisotropy in birefringent biological tissues with polarization-sensitive optical coherence tomography
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用偏振敏感光学相干断层扫描分析双折射生物组织光学各向异性的理论框架

DOI:
10.1117/12.889208
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发表时间:
2011
期刊:
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通讯作者:
Kasaragod D
Kasaragod D
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作者:
Kasaragod D

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偏振敏感光学相干层析成像(PSOCT)是一种重要的非侵入性光学成像工具,可用于双折射生物组织的研究。利用时域PSOCT(TD-PSOCT)系统研究了关节软骨中胶原纤维的复杂三维结构体系,并与三层软骨模型进行了比较。从牛软骨样品的可变入射角(VIA)实验中获得的PSOCT结果被认为是与基于扫描电子显微镜研究提出的板层软骨模型一致。这显示了PSOCT-VIA技术的潜在用途,以获得关于软骨中胶原纤维的复杂3D结构的深度信息。必须进行进一步的研究,以绘制出软骨不同部位的深度延迟信息,这可以建立这种方法的一般有效性。
Polarization-sensitive optical coherence tomography (PSOCT) has established itself as an important non-invasive optical imaging tool to study the birefringent biological tissues. The complex 3D structure architecture of the collagen fibers in articular cartilage is investigated using a time domain PSOCT (TD-PSOCT) system and the depth-wise cumulative retardance profiles obtained are compared with a three layer cartilage model. The PSOCT result obtained from the variable incidence angle (VIA) experiment of bovine cartilage sample is found to be consistent with the proposed lamellar cartilage model based on scanning electron microscope studies. This shows potential use of PSOCT–VIA technique to obtain depth-wise information about the complex 3D architecture of collagen fibers in the cartilage. Further studies would have to be carried out to map out depth wise retardance information at different sites of the cartilage, which could establish the general validity of this approach.
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