The carcinogenic liver fluke, Clonorchis sinensis: new assembly, reannotation and analysis of the genome and characterization of tissue transcriptomes.
The carcinogenic liver fluke, Clonorchis sinensis: new assembly, reannotation and analysis of the genome and characterization of tissue transcriptomes.
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致癌肝吸虫，华支睾吸虫：基因组的新组装、重新注释和分析以及组织转录组的表征
DOI：
10.1371/journal.pone.0054732
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Huang Y;Chen W;Wang X;Liu H;Chen Y;Guo L;Luo F;Sun J;Mao Q;Liang P;Xie Z;Zhou C;Tian Y;Lv X;Huang L;Zhou J;Hu Y;Li R;Zhang F;Lei H;Li W;Hu X;Liang C;Xu J;Li X;Yu X
Clonelis sinensis（C. sinensis）是一种重要的食源性寄生虫，寄生于肝内胆管并引起华支睾吸虫病，引起了公共卫生领域和科学研究界的兴趣。目的：了解该虫的迁飞、寄生及致病机理。在分子水平上，本研究开发了一种升级的基因组组装和注释，通过测序配对末端和配对文库。我们还对多个C.中华绒螯蟹组织（吸盘、肌肉、卵巢和精巢）。编码刺激反应和肌肉相关发育分子的基因在口吸盘中大量表达。与其他物种相比，编码促进胆固醇识别和转运的分子的基因在基因组中以高拷贝数存在，并且在口吸盘中高度表达。编码脂肪酸、葡萄糖、氨基酸和氧转运蛋白的基因也高度表达，沿着参与代谢这些底物的其他分子。参与能量代谢途径的所有基因，包括脂肪酸的β-氧化、柠檬酸循环、氧化磷酸化和富马酸盐还原，在成体中表达。最后，我们也提供了有价值的见解，发病机制的过程中，通过表征分泌组的C。sinensis。对升级后的基因组和组织转录组的表征和精细分析不仅形成了一个详细的和基本的C。同时也为深入研究冬虫夏草的生理和发病机制提供了新的思路。sinensis。我们预计，这项工作将有助于制定创新的战略，预防和控制华支睾吸虫病。
Clonorchis sinensis (C. sinensis), an important food-borne parasite that inhabits the intrahepatic bile duct and causes clonorchiasis, is of interest to both the public health field and the scientific research community. To learn more about the migration, parasitism and pathogenesis of C. sinensis at the molecular level, the present study developed an upgraded genomic assembly and annotation by sequencing paired-end and mate-paired libraries. We also performed transcriptome sequence analyses on multiple C. sinensis tissues (sucker, muscle, ovary and testis). Genes encoding molecules involved in responses to stimuli and muscle-related development were abundantly expressed in the oral sucker. Compared with other species, genes encoding molecules that facilitate the recognition and transport of cholesterol were observed in high copy numbers in the genome and were highly expressed in the oral sucker. Genes encoding transporters for fatty acids, glucose, amino acids and oxygen were also highly expressed, along with other molecules involved in metabolizing these substrates. All genes involved in energy metabolism pathways, including the β-oxidation of fatty acids, the citrate cycle, oxidative phosphorylation, and fumarate reduction, were expressed in the adults. Finally, we also provide valuable insights into the mechanism underlying the process of pathogenesis by characterizing the secretome of C. sinensis. The characterization and elaborate analysis of the upgraded genome and the tissue transcriptomes not only form a detailed and fundamental C. sinensis resource but also provide novel insights into the physiology and pathogenesis of C. sinensis. We anticipate that this work will aid the development of innovative strategies for the prevention and control of clonorchiasis.
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影响因子：
4
作者：
Bateman, Andrew;Bennett, Hugh P. J.
通讯作者：
Bennett, Hugh P. J.
影响因子：
1.5
作者：
Hu, Fengyu;Hu, Xuchu;Yu, Xinbing
通讯作者：
Yu, Xinbing
影响因子：
4.4
作者：
Garg G;Ranganathan S
通讯作者：
Ranganathan S
影响因子：
1.5
作者：
COUSIN, C;DORSEY, C;OFORI, K
通讯作者：
OFORI, K
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影响因子：
2
作者：
Huang, Yan;Li, Wenfang;Yu, Xinbing
通讯作者：
Yu, Xinbing