Evaluation of polymethine dyes as potential probes for near infrared fluorescence imaging of tumors: part - 1.
Evaluation of polymethine dyes as potential probes for near infrared fluorescence imaging of tumors: part - 1.
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DOI：
10.7150/thno.5922
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发表时间：
2013
期刊：
影响因子：
12.4
通讯作者：
Pandey RK
中科院分区：
文献类型：
--
作者：
James NS;Chen Y;Joshi P;Ohulchanskyy TY;Ethirajan M;Henary M;Strekowsk L;Pandey RK
近红外（NIR）有机染料对于许多生物医学应用（包括体内光学成像）已经变得重要。NIR荧光染料与光敏分子（光敏剂）的缀合对于癌症的NIR荧光图像引导的光动力疗法（PDT）具有很强的潜力。因此，我们有兴趣研究一系列杂环多甲川染料的生物物理性质、体内肿瘤亲和性和荧光成像潜力，然后将其与某些PDT试剂缀合。对于我们目前的研究，我们选择了一系列的对称聚甲炔染料含有各种双-N-取代的吲哚或苯并吲哚部分连接的线性共轭与和没有稠合取代的环己烯环。在C-末端位置的N-烷基侧链用磺酸、羧酸、甲酯或羟基官能化。虽然，在所研究的母体花青染料中，市售的具有环状氯代环己烯部分的花青染料IR 783（3）（双吲哚-N-丁基磺酸盐）-聚甲川染料基于其光谱特性（λAbs=782 nm，λ FI =810 nm，ε = 261，000 M-1cm-1，Φ FI ≤ 0.08）和肿瘤亲和性显示出最佳的荧光成像能力。除了3之外，还选择了母体染料IR 820和Cypate（6），并通过引入所需的官能团进行进一步修饰，这可以使花青染料与我们实验室开发的有效光敏剂HPPH进一步缀合。合成和生物学研究（肿瘤成像和PDT）所产生的双功能共轭物中讨论了后续文件（本研究的第2部分）。
Near-infrared (NIR) organic dyes have become important for many biomedical applications, including in vivo optical imaging. Conjugation of NIR fluorescent dyes to photosensitizing molecules (photosensitizers) holds strong potential for NIR fluorescence image guided photodynamic therapy (PDT) of cancer. Therefore, we were interested in investigating the photophysical properties, in vivo tumor-affinity and fluorescence imaging potential of a series of heterocyclic polymethine dyes, which could then be conjugated to certain PDT agents. For our present study, we selected a series of symmetrical polymethine dyes containing a variety of bis-N-substituted indole or benzindole moieties linked by linear conjugation with and without a fused substituted cyclohexene ring. The N-alkyl side chain at the C-terminal position was functionalized with sulfonic, carboxylic acid, methyl ester or hydroxyl groups. Although, among the parent cyanine dyes investigated, the commercially available, cyanine dye IR783 (3) (bis-indole-N-butylsulfonate)-polymethine dye with a cyclic chloro-cyclohexene moiety showed best fluorescence-imaging ability, based on its spectral properties (λAbs=782 nm, λFl=810 nm, ε = 261,000 M-1cm-1, ΦFl≈0.08) and tumor affinity. In addition to 3, parent dyes IR820 and Cypate (6) were also selected and subjected to further modifications by introducing desired functional groups, which could enable further conjugation of the cyanine dyes to an effective photosensitizer HPPH developed in our laboratory. The synthesis and biological studies (tumor-imaging and PDT) of the resulting bifunctional conjugates are discussed in succeeding paper (Part-2 of this study).
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影响因子：
2.4
作者：
Nava, Hector R.;Allamaneni, Shyam S.;Dougherty, Thomas J.;Cooper, Michele T.;Tan, Wei;Wilding, Gregory;Henderson, Barbara W.
通讯作者：
Henderson, Barbara W.
影响因子：
4.7
作者：
Moon, WK;Lin, YH;Tung, CH
通讯作者：
Tung, CH
影响因子：
11.2
作者：
Veiseh, Mandana;Gabikian, Patrik;Olson, James M.
通讯作者：
Olson, James M.
影响因子：
15
作者：
DEMAS, JN;HARRIS, EW;MCBRIDE, RP
通讯作者：
MCBRIDE, RP
影响因子：
11.5
作者：
Kaijzel, Eric L.;van der Pluijm, Gabri;Lowik, Clemens W. G. M.
通讯作者：
Lowik, Clemens W. G. M.