Gastrodin protects H9c2 cardiomyocytes against oxidative injury by ameliorating imbalanced mitochondrial dynamics and mitochondrial dysfunction.
Gastrodin protects H9c2 cardiomyocytes against oxidative injury by ameliorating imbalanced mitochondrial dynamics and mitochondrial dysfunction.
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DOI：
10.1038/s41401-020-0382-x
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发表时间：
2020-10
影响因子：
8.2
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Cheng QQ;Wan YW;Yang WM;Tian MH;Wang YC;He HY;Zhang WD;Liu X
天麻是中药天麻的主要生物活性成分，被广泛用于治疗神经系统疾病和心脑血管疾病。本研究发现，GAS对H9c2细胞缺血再灌注（IR）样损伤的保护作用与降低氧化应激有关。此外，GAS对H2O2诱导的H9c2细胞氧化损伤具有保护作用。GAS在20、50和100 μM浓度下处理4 h，可显著改善400 μM H2O2处理3 h后H9c2细胞活力下降和凋亡增加的现象。此外，我们发现H2O2处理导致线粒体断裂，线粒体网络、足迹和管状长度显著减少；H2O2处理强烈抑制线粒体呼吸；H2O2处理导致线粒体融合因子Mfn2和Opa1的表达降低，线粒体裂变因子Fis1的表达升高。这些变化在h2o2处理的H9c2细胞中都可以通过GAS预处理得到改善。此外，我们发现GAS预处理增强了H2O2处理下Nrf2的核易位。Nrf2表达下调可消除GAS对h2o2处理的H9c2细胞的保护作用。我们的研究结果表明，GAS可能通过增加Nrf2的核易位，调节线粒体动力学，维持线粒体的结构和功能来保护H9c2心肌细胞免受氧化损伤。
Gastrodin (GAS) is the main bioactive component of Tianma, a traditional Chinese medicine widely used to treat neurological disorders as well as cardio- and cerebrovascular diseases. In the present study, the protective effects of GAS on H9c2 cells against ischemia–reperfusion (IR)-like injury were found to be related to decreasing of oxidative stress. Furthermore, GAS could protect H9c2 cells against oxidative injury induced by H2O2. Pretreatment of GAS at 20, 50, and 100 μM for 4 h significantly ameliorated the decrease in cell viability and increase in apoptosis of H9c2 cells treated with 400 μM H2O2 for 3 h. Furthermore, we showed that H2O2 treatment induced fragmentation of mitochondria and significant reduction in networks, footprint, and tubular length of mitochondria; H2O2 treatment strongly inhibited mitochondrial respiration; H2O2 treatment induced a decrease in the expression of mitochondrial fusion factors Mfn2 and Opa1, and increase in the expression of mitochondrial fission factor Fis1. All these alterations in H2O2-treated H9c2 cells could be ameliorated by GAS pretreatment. Moreover, we revealed that GAS pretreatment enhanced the nuclear translocation of Nrf2 under H2O2 treatment. Knockdown of Nrf2 expression abolished the protective effects of GAS on H2O2-treated H9c2 cells. Our results suggest that GAS may protect H9c2 cardiomycytes against oxidative injury via increasing the nuclear translocation of Nrf2, regulating mitochondrial dynamics, and maintaining the structure and functions of mitochondria.
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影响因子：
10.8
作者：
Chen, Le;Gong, Qizhi;Knowlton, Anne A.
通讯作者：
Knowlton, Anne A.
影响因子：
3.1
作者：
de Oliveira, Marcos Roberto;Brasil, Flavia Bittencourt;Furstenau, Cristina Ribas
通讯作者：
Furstenau, Cristina Ribas
DOI：
10.2174/1874091x01206010056
发表时间：
2012
期刊：
The open biochemistry journal
影响因子：
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作者：
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通讯作者：
Heese K
天麻异钩藤碱通过靶向 ERK1/2 和 GSK-3β 途径协同抑制 MPP 诱导的 SH-SY5Y 细胞氧化应激：参与 Nrf2 核易位
影响因子：
5
作者：
Li, Qiang;Niu, Chengu;Dong, Miaoxian
通讯作者：
Dong, Miaoxian
DOI：
10.1080/13510002.2018.1492766
发表时间：
2018-12
期刊：
Redox report : communications in free radical research
影响因子：
--
作者：
Garcia I;Innis-Whitehouse W;Lopez A;Keniry M;Gilkerson R
通讯作者：
Gilkerson R