Blood Flow Decrease Induces Apoptosis of Endothelial Cells in Previously Dilated Arteries Resulting From Chronic High Blood Flow
Blood Flow Decrease Induces Apoptosis of Endothelial Cells in Previously Dilated Arteries Resulting From Chronic High Blood Flow
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血流量减少会导致先前因慢性高血流量而扩张的动脉内皮细胞凋亡
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
H. Masuda
中科院分区：
文献类型：
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作者：
E. Sho;M. Sho;T. Singh;Chengpei Xu;C. Zarins;H. Masuda
我们研究了动脉狭窄过程中血管内皮细胞对血流减少的反应。闭合动静脉瘘(AVF)可导致颈动脉血流量减少，该瘘已在兔体内建立了28天。应用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法，结合激光扫描共聚焦显微镜、透射和扫描电子显微镜，观察AVF闭塞后1、3、7、21d颈动脉内皮细胞的凋亡情况。AVF封堵后动脉管腔直径缩小36%，第21天内皮细胞数量较封堵前减少45%。内皮细胞凋亡率1d达高峰，3d达高峰(381.3±87.1cell/mm2)，7d下降。扫描电子显微镜下可见不规则突起，透射电子显微镜下可见细胞核碎裂。凋亡细胞主要位于内皮下，偶见于血管内皮细胞和血管内皮细胞内。结果提示，血管内皮细胞的凋亡可能在血流减少所致的动脉重塑中起一定作用。
We investigated apoptosis of endothelial cells during the arterial narrowing process in response to reduction in flow. The decrease in flow was created in the carotid artery by closure of an arteriovenous fistula (AVF), which had been established for 28 days in rabbits. The endothelial cell apoptosis in the carotid artery was studied at 1, 3, 7, and 21 days of flow reduction after closure of the AVF by use of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) with laser scanning confocal microscopy and transmission and scanning electron microscopy. After AVF closure, arterial lumen diameter was reduced by 36%, and compared with endothelial cells before the closure, the number of endothelial cells was decreased by 45% at 21 days. Endothelial cell apoptosis was observed at 1 day, peaked at 3 days (381.3±87.1 cells per square millimeter), and decreased at 7 days. These cells had irregular protrusions under scanning electron microscopy and were characterized by fragmented nuclei under transmission electron microscopy. Apoptotic cells were mainly beneath the endothelium and were occasionally within smooth muscle cells and endothelial cells. The results suggest that apoptosis of endothelial cells may play a role in the arterial remodeling in response to a reduction in flow.
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影响因子：
--
作者：
COOKE, JP;STAMLER, J;LOSCALZO, J
通讯作者：
LOSCALZO, J
DOI：
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发表时间：
1995-08
期刊：
The American journal of pathology
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
20.3
作者：
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通讯作者：
T. Bombeli;A. Karsan;J. Tait;J. Harlan