Properties of transgenic strains of Drosophila melanogaster containing I transposable elements from Drosophila teissieri.
Properties of transgenic strains of Drosophila melanogaster containing I transposable elements from Drosophila teissieri.
复制标题
含有来自 Drosophila teissieri 的 I 转座元件的黑腹果蝇转基因菌株的特性。
DOI：
10.1017/s0016672300031177
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
A. Bucheton
中科院分区：
文献类型：
--
作者：
C. Vaury;A. Pélisson;P. Abad;A. Bucheton
I 因子是果蝇的转座元件，类似于哺乳动物 LINE，通过 RNA 中间体的逆转录进行转座，并负责杂交发育不全的 I-R 系统。该物种中有两类菌株：诱导菌株，在染色体臂的不同位点含有约 15 个 I 元素；反应菌株，缺乏活性 I 因子。 I 元素存在于各种果蝇物种中。当通过 P 元件介导的转化将来自果蝇的潜在功能性 I 因子引入黑腹果蝇反应株中时，它们可以转位。我们研究了黑腹果蝇菌株的特性，其中引入了来自果蝇 teissieri 的 I 因子（名为 Itei）。当携带 Itei 的雄性与反应性雌性杂交时，就会产生典型的杂交发育不全。然而，产生不良性状似乎需要多于一个该元素的拷贝，而果蝇仅一个 I 因子似乎就足够了。通过内配杂交维持的转化系中 Itei 的拷贝数迅速增加，并稳定在与诱导菌株中观察到的值相似的值。由于 Drosophila teissieri 含有的拷贝数比 Drosophila melanogaster 菌株少得多，这表明 I 元件的拷贝数不仅仅由元件本身中存在的序列调节。
I factors are transposable elements of Drosophila melanogaster similar to mammalian LINEs, that transpose by reverse transcription of an RNA intermediate and are responsible for the I-R system of hybrid dysgenesis. There are two categories of strains in this species: inducer, that contain about 15 I elements at the various sites on chromosomal arms, and reactive, that lack active I factors. I elements occur in various Drosophila species. Potentially functional I factors from Drosophila teissieri can transpose when introduced by P-element-mediated transformation in a reactive strain of Drosophila melanogaster. We have studied the properties of Drosophila melanogaster strains into which such an I factor from Drosophila teissieri, named Itei, was introduced. Typical hybrid dysgenesis is produced when males carrying Itei are crossed with reactive females. However, more than one copy of the element seems necessary to produce dysgenic traits, whereas only one I factor of Drosophila melanogaster seems to be sufficient. The copy number of Itei in transformed lines maintained by endogamous crosses increases rapidly and stabilizes at values similar to those observed in inducer strains. As Drosophila teissieri contains much fewer copies than the Drosophila melanogaster strains, this suggests that the copy number of I elements is not simply regulated by sequences present in the element itself.
非自主P元件对果蝇杂交发育不全的影响。
影响因子：
3.3
作者：
Simmons,MJ;Raymond,JD;Boedigheimer,MJ;Zunt,JR
通讯作者：
Zunt,JR