In vivo functional analysis of the Dicistroviridae intergenic region internal ribosome entry sites.
In vivo functional analysis of the Dicistroviridae intergenic region internal ribosome entry sites.
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DOI：
10.1093/nar/gkr427
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发表时间：
2011-09-01
影响因子：
14.9
通讯作者：
Thompson SR
中科院分区：
文献类型：
--
作者：
Hertz MI;Thompson SR
Some viral and cellular messages use an alternative mechanism to initiate protein synthesis that involves internal recruitment of the ribosome to an internal ribosome entry site (IRES). The Dicistroviridae intergenic regions (IGR) have been studied as model IRESs to understand the mechanism of IRES-mediated translation. In this study, the in vivo activity of IGR IRESs were compared. Our analysis demonstrates that Class I and II IGR IRESs have comparable translation efficiency in yeast and that Class II is significantly more active in mammalian cells. Furthermore, while Class II IGR IRES activity was enhanced in yeast grown at a higher temperature, temperature did not affect IGR IRES activity in mammalian cells. This suggests that Class II IRESs may not function optimally with yeast ribosomes. Examination of chimeric IGR IRESs, established that the IRES strength and temperature sensitivity are mediated by the ribosome binding domain. In addition, the sequence of the first translated codon is also an important determinant of IRES activity. Our findings provide us with a comprehensive overview of IGR IRES activities and allow us to begin to understand the differences between Classes I and II IGR IRESs.
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影响因子：
3.7
作者：
Hertz MI;Thompson SR
通讯作者：
Thompson SR
影响因子：
14.9
作者：
Muhs M;Yamamoto H;Ismer J;Takaku H;Nashimoto M;Uchiumi T;Nakashima N;Mielke T;Hildebrand PW;Nierhaus KH;Spahn CM
通讯作者：
Spahn CM
影响因子：
4.5
作者：
Costantino, D;Kieft, JS
通讯作者：
Kieft, JS
影响因子：
4.5
作者：
Hatakeyama, Y;Shibuya, N;Nakashima, N
通讯作者：
Nakashima, N
影响因子：
3.8
作者：
Mari, J;Poulos, BT;Bonami, JR
通讯作者：
Bonami, JR