From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae.
From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae.
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DOI：
10.1186/s13068-016-0600-4
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发表时间：
2016
影响因子：
6.3
通讯作者：
Kondo A
中科院分区：
文献类型：
--
作者：
Ishii J;Okazaki F;Djohan AC;Hara KY;Asai-Nakashima N;Teramura H;Andriani A;Tominaga M;Wakai S;Kahar P;Yopi;Prasetya B;Ogino C;Kondo A
甘露糖是针叶树种中最大的半纤维素部分，也是各种植物中碳水化合物的储存库。然而，在可持续生物燃料发展中，甘露聚糖作为可持续资源的利用一直不太先进。在酵母细胞表面展示技术的基础上，我们构建了一株共展示β-甘露聚糖酶和β-甘露糖苷酶的重组酿酒酵母菌株，该菌株有望促进以甘露聚糖为生物质来源的乙醇发酵。亲本酿酒酵母以单体糖的形式吸收甘露糖和葡萄糖，从甘露糖中生产乙醇。我们构建了表达β-甘露聚糖酶和β-甘露糖苷酶的酵母菌株，免疫荧光染色和酶活性测定证实这两种酶在细胞表面共展示。构建的酵母细胞成功地降解了1，4-β-d-甘露聚糖，并通过同化产生的甘露糖来生产乙醇，而不需要外加酶。此外，构建的菌株在第三批重复发酵过程中，1，4-β-d-甘露聚糖连续发酵生产乙醇。此外，构建的菌株利用象牙坚果甘露聚糖生产乙醇，通过对底物进行氢氧化钠预处理，提高了乙醇的产量。我们成功地在酵母细胞表面展示了β-甘露聚糖酶和β-甘露糖苷酶。我们的结果清楚地证明了β-甘露聚糖酶和β-甘露糖苷酶共展示菌株在甘露聚糖生物质酒精发酵中的应用。因此，在酵母细胞表面共连接β-甘露聚糖酶和β-甘露糖苷酶为酵母发酵生产生物乙醇和其他生物化学物质提供了一个强大的平台技术。本文的在线版本(doi：10.1186/s13068-0160600-4)包含补充材料，授权用户可以使用。
Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays β-mannanase and β-mannosidase; this strain is expected to facilitate ethanol fermentation using mannan as a biomass source. Parental yeast S. cerevisiae assimilated mannose and glucose as monomeric sugars, producing ethanol from mannose. We constructed yeast strains that express tethered β-mannanase and β-mannosidase; co-display of the two enzymes on the cell surface was confirmed by immunofluorescence staining and enzyme activity assays. The constructed yeast cells successfully hydrolyzed 1,4-β-d-mannan and produced ethanol by assimilating the resulting mannose without external addition of enzymes. Furthermore, the constructed strain produced ethanol from 1,4-β-d-mannan continually during the third batch of repeated fermentation. Additionally, the constructed strain produced ethanol from ivory nut mannan; ethanol yield was improved by NaOH pretreatment of the substrate. We successfully displayed β-mannanase and β-mannosidase on the yeast cell surface. Our results clearly demonstrate the utility of the strain co-displaying β-mannanase and β-mannosidase for ethanol fermentation from mannan biomass. Thus, co-tethering β-mannanase and β-mannosidase on the yeast cell surface provides a powerful platform technology for yeast fermentation toward the production of bioethanol and other biochemicals from lignocellulosic materials containing mannan components. The online version of this article (doi:10.1186/s13068-016-0600-4) contains supplementary material, which is available to authorized users.
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影响因子：
4.1
作者：
Kondo, Takashi;Tezuka, Hironori;Kondo, Akihiko
通讯作者：
Kondo, Akihiko
影响因子：
11.4
作者：
Matano, Yuki;Hasunuma, Tomohisa;Kondo, Akihiko
通讯作者：
Kondo, Akihiko
影响因子：
6.3
作者：
Inokuma K;Hasunuma T;Kondo A
通讯作者：
Kondo A
影响因子：
1.6
作者：
Setati, ME;Ademark, P;Stålbrand, H
通讯作者：
Stålbrand, H
DOI：
10.1016/s0021-9673(01)98394-3
发表时间：
1960-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
BAILEY, RW;BOURNE, EJ
通讯作者：
BOURNE, EJ