Antioxidant activity and components of a traditional Chinese medicine formula consisting of Crataegus pinnatifida and Salvia miltiorrhiza.
Antioxidant activity and components of a traditional Chinese medicine formula consisting of Crataegus pinnatifida and Salvia miltiorrhiza.
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DOI：
10.1186/1472-6882-13-99
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发表时间：
2013-05-10
影响因子：
--
通讯作者：
Yang B
中科院分区：
文献类型：
--
作者：
Chen CY;Li H;Yuan YN;Dai HQ;Yang B
本研究的目的是评价抗氧化活性，并确定由山楂（山楂的果实）组成的传统中药配方的抗氧化成分。变种N.E.B.少校SZ）和丹参（丹参的根，DS）。这种药被广泛用于治疗心血管疾病。分析了从SZ、DS和配方（SZ+DS）中提取和分级的12个样品。采用高效液相色谱法测定了8种酚类化合物的含量。采用氧自由基吸收能力（ORAC）法和1，1-二苯基-2-苦基肼法对样品及15种酚类化合物的抗氧化活性进行了研究。对中草药样品的抗氧化活性与其酚类成分进行了相关性分析。所有SZ+DS样品中的主要酚类成分均为丹参酮酸B，与其他酚类化合物相比，其表现出较强的抗氧化活性（ORAC值：16.73 ± 2.53，IC 50值：8.80 ± 0.06 μM）。对于所有样品，有一个积极的关系，其总酚类成分和它们的抗氧化活性。酚类化合物是中药样品的生物活性成分，丹参素酸B被鉴定为SZ+DS配方中的主要生物活性成分。
The aims of this study were to evaluate the antioxidant activity and to identify the antioxidant components of a traditional Chinese medicine formula consisting of a combination of Shanzha (the fruit of Crataegus pinnatifida Bge. var. major N.E.Br., SZ) and Danshen (the root of Salvia miltiorrhiza Bge., DS). This medicine is extensively used to treat cardiovascular disease. Twelve samples extracted and fractionated from SZ, DS and the formula (SZ+DS) were analyzed. The concentrations of eight phenolic compounds were determined by high performance liquid chromatography. Oxygen radical absorbance capacity (ORAC) assay and 1,1-diphenyl-2-picrylhydrazyl assay were conducted to explore the antioxidant activities of the samples and of the 15 phenolic compounds detected. Correlation analysis of the antioxidant activity of herb samples and their phenolic components was performed. The main phenolic component in all SZ+DS samples was salvianolic acid B, which exhibited strong antioxidant activity (ORAC value: 16.73 ± 2.53, IC50 value: 8.80 ± 0.06 μM) compared with the other phenolic compounds. For all samples, there was a positive relationship between their total phenolic components and their antioxidant activities. Phenolic compounds were the bioactive components of the herb samples, and salvianolic acid B was identified as the main bioactive compound in the SZ+DS formula.
影响因子：
4.3
作者：
Zhao, Guang-Rong;Zhang, Heng-Ming;Zhao, Li-Bin
通讯作者：
Zhao, Li-Bin
影响因子：
6.1
作者：
Prior, RL;Wu, XL;Schaich, K
通讯作者：
Schaich, K
影响因子：
4.8
作者：
Ou, BX;Huang, DJ;Flanagan, JA
通讯作者：
Flanagan, JA