Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing.
Lycopene Is Enriched in Tomato Fruit by CRISPR/Cas9-Mediated Multiplex Genome Editing.
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通过 CRISPR/Cas9 介导的多重基因组编辑，番茄果实中富含番茄红素
DOI：
10.3389/fpls.2018.00559
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Li X;Wang Y;Chen S;Tian H;Fu D;Zhu B;Luo Y;Zhu H
考虑到番茄红素积累对果实视觉和功能特性的积极影响，许多研究都集中在培育番茄红素积累增强的番茄植株上。在这项研究中，我们采用了双向策略：促进番茄红素的生物合成，同时抑制番茄红素向β-和α-胡萝卜素的转化。番茄红素的积累是通过敲除一些与类胡萝卜素代谢途径相关的基因来促进的。最后，通过农杆菌介导的转化，通过CRISPR/Cas9系统选择5个基因在基因组中进行编辑。我们的研究结果表明，CRISPR/Cas9是一种位点特异性基因组编辑技术，可以在多个感兴趣的基因中进行高效的靶突变。令人惊讶的是，经过基因组编辑的番茄果实中番茄红素含量成功增加到约5.1倍。纯合突变稳定地传递给后代。综上所述，我们的研究结果表明，CRISPR/Cas9系统可以显著提高番茄果实中番茄红素的含量，具有效率高、脱靶突变少、遗传稳定等优点。
Numerous studies have been focusing on breeding tomato plants with enhanced lycopene accumulation, considering its positive effects of fruits on the visual and functional properties. In this study, we used a bidirectional strategy: promoting the biosynthesis of lycopene, while inhibiting the conversion from lycopene to β- and α-carotene. The accumulation of lycopene was promoted by knocking down some genes associated with the carotenoid metabolic pathway. Finally, five genes were selected to be edited in genome by CRISPR/Cas9 system using Agrobacterium tumefaciens-mediated transformation. Our findings indicated that CRISPR/Cas9 is a site-specific genome editing technology that allows highly efficient target mutagenesis in multiple genes of interest. Surprisingly, the lycopene content in tomato fruit subjected to genome editing was successfully increased to about 5.1-fold. The homozygous mutations were stably transmitted to subsequent generations. Taken together, our results suggest that CRISPR/Cas9 system can be used for significantly improving lycopene content in tomato fruit with advantages such as high efficiency, rare off-target mutations, and stable heredity.
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影响因子：
4.6
作者：
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通讯作者：
Bramley, PM