Large-scale and high-confidence proteomic analysis of human seminal plasma.
Large-scale and high-confidence proteomic analysis of human seminal plasma.
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DOI：
10.1186/gb-2006-7-5-r40
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发表时间：
2006
期刊：
影响因子：
12.3
通讯作者：
Mann M
中科院分区：
文献类型：
--
作者：
Pilch B;Mann M
精液中923种蛋白质的高置信度鉴定提供了一个在受精中具有潜在作用的蛋白质清单。质谱（MS）技术的发展，现在允许调查非常复杂的蛋白质混合物，从亚细胞结构到组织。体液也是蛋白质组学分析的流行目标，因为它们具有发现生物标志物的潜力。精浆尚未受到蛋白质组学界的广泛关注，但其特征可以为几乎所有涉及人类精子的研究提供参考。这种液体对于精子的存活和它们在女性生殖道中的成功旅程是必不可少的。在这里，我们报告的高置信度鉴定923蛋白质精液从一个单一的个人。傅立叶变换MS实现了百万分之一的质量准确度，并且两个连续的MS片段化阶段允许甚至通过单个肽来置信地鉴定蛋白质。GoMiner分析注释了三分之二的精液蛋白质组，并揭示了大量的细胞外蛋白质，包括许多蛋白酶。其他蛋白质来源于雄性附腺，在精子存活中起重要作用。精浆含量的这种高置信度表征提供了在受精中具有潜在作用的蛋白质的清单。当与定量蛋白质组学方法相结合时，它应该对受精、男性不育、前列腺和睾丸癌的研究有用。
The high-confidence identification of 923 proteins in seminal fluid provides an inventory of proteins with potential roles in fertilization. The development of mass spectrometric (MS) techniques now allows the investigation of very complex protein mixtures ranging from subcellular structures to tissues. Body fluids are also popular targets of proteomic analysis because of their potential for biomarker discovery. Seminal plasma has not yet received much attention from the proteomics community but its characterization could provide a future reference for virtually all studies involving human sperm. The fluid is essential for the survival of spermatozoa and their successful journey through the female reproductive tract. Here we report the high-confidence identification of 923 proteins in seminal fluid from a single individual. Fourier transform MS enabled parts per million mass accuracy, and two consecutive stages of MS fragmentation allowed confident identification of proteins even by single peptides. Analysis with GoMiner annotated two-thirds of the seminal fluid proteome and revealed a large number of extracellular proteins including many proteases. Other proteins originated from male accessory glands and have important roles in spermatozoan survival. This high-confidence characterization of seminal plasma content provides an inventory of proteins with potential roles in fertilization. When combined with quantitative proteomics methodologies, it should be useful for studies of fertilization, male infertility, and prostatic and testicular cancers.
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影响因子：
7
作者：
Olsen, JV;Ong, SE;Mann, M
通讯作者：
Mann, M
影响因子：
2.8
作者：
Fung, KYC;Glode, LM;Duncan, MW
通讯作者：
Duncan, MW
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
DOI：
10.1073/pnas.0405549101
发表时间：
2004-09-14
影响因子：
11.1
作者：
Olsen, JV;Mann, M
通讯作者：
Mann, M
影响因子：
--
作者：
Lwaleed, BA;Greenfield, R;Cooper, AJ
通讯作者：
Cooper, AJ