Genome-wide identification, characterization and expression profiles of the CCD gene family in Gossypium species
Genome-wide identification, characterization and expression profiles of the CCD gene family in Gossypium species
复制标题
棉属 CCD 基因家族的全基因组鉴定、表征和表达谱
DOI：
10.1007/s13205-021-02805-9
复制
发表时间：
2021-05
期刊：
影响因子：
2.8
通讯作者：
Yuchen Miao
中科院分区：
文献类型：
--
作者：
Shulin Zhang;Yutao Guo;Yanqi Zhang;Jinggong Guo;Kun Li;Weiwei Fu;Zhenzhen Jia;Weiqiang Li;Lam‑Son Phan Tran;Kun‑Peng Jia;Yuchen Miao
类胡萝卜素裂解双加氧酶（CCDs）是一类催化从类胡萝卜素到类胡萝卜素选择性氧化裂解的酶，类胡萝卜素是合成类维生素a、植物激素脱落酸（ABA）和独角酯内酯等生物重要分子所必需的酶。此外，ccd在植物的生物和非生物胁迫响应中发挥着重要作用。迄今为止，对重要经济作物棉花（Gossypiumspp.）中cdg基因家族的全面表征仍然缺失。在这里，我们进行了全基因组分析，鉴定了33、31、16和15个ccd基因来自两个异源四倍体棉属植物G。hirsutumandG。巴巴多斯，和两个二倍体棉属，G。arboreumandG。raimondii就分别。通过系统进化树分析，将棉花ccds划分为ccd1、CCD4、CCD7、CCD8、9顺式环氧类胡萝卜素双加氧酶（NCED）和扎辛酮合成酶（ZAS）亚家族6个亚群。进化分析表明，纯化选择主导了这些基因的进化。hirsutumandG。取得。ccd启动子的预测作用元件[j]。多毛主要与光、应激和激素反应有关。对ghccds的转录组学分析表明，不同的ghccds表现出不同的表达模式，在大多数组织中普遍表达；此外，不同的非生物胁迫对GhCCDs具有特异性诱导作用。定量反转录PCR （qRT-PCR）证实了热胁迫、盐度、聚乙二醇（PEG）和ABA对ghccds的诱导作用。综上所述，ccdgene家族的生物信息学和表达分析为参与调控非生物胁迫提供了证据，为深入研究其生物学功能提供了有用的信息。分子。补充资料：在线版本提供补充资料，网址为10.1007/s13205-021-02805-9。
Carotenoid cleavage dioxygenases (CCDs) are a group of enzymes that catalyze the selective oxidative cleavage steps from carotenoids to apocarotenoids, which are essential for the synthesis of biologically important molecules such as retinoids, and the phytohormones abscisic acid (ABA) and strigolactones. In addition, CCDs play important roles in plant biotic and abiotic stress responses. Till now, a comprehensive characterization of theCCDgene family in the economically important crop cotton (Gossypiumspp.) is still missing. Here, we performed a genome-wide analysis and identified 33, 31, 16 and 15CCDgenes from two allotetraploidGossypiumspecies,G. hirsutumandG. barbadense, and two diploidGossypiumspecies,G. arboreumandG. raimondii, respectively. According to the phylogenetic tree analysis, cottonCCDs are classified as six subgroups includingCCD1,CCD4,CCD7,CCD8,nine-cis-epoxycarotenoid dioxygenase(NCED) and zaxinone synthase(ZAS) sub-families. Evolutionary analysis shows that purifying selection dominated the evolution of these genes inG. hirsutumandG. barbadense. Predictedcis-acting elements in 2 kb promoters ofCCDs inG. hirsutumare mainly involved in light, stress and hormone responses. The transcriptomic analysis ofGhCCDs showed that differentGhCCDs displayed diverse expression patterns and were ubiquitously expressed in most tissues; moreover,GhCCDs displayed specific inductions by different abiotic stresses. Quantitative reverse-transcriptional PCR (qRT-PCR) confirmed the induction ofGhCCDs by heat stress, salinity, polyethylene glycol (PEG) and ABA application. In summary, the bioinformatics and expression analysis ofCCDgene family provide evidence for the involvement in regulating abiotic stresses and useful information for in-depth studies of their biological functions inG. hirsutum.Supplementary information:The online version contains supplementary material available at 10.1007/s13205-021-02805-9.
登录
查看更多内容
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
非生物胁迫处理期间大豆类胡萝卜素加氧酶基因的全基因组鉴定和转录分析
影响因子：
2.8
作者：
Wang, Chun-E;Fei, Yun-Yan;Gai, Jun-Yi;Zhao, Tuan-Jie
通讯作者：
Zhao, Tuan-Jie
影响因子：
2.1
作者：
P. Sexton;C. J. Gerard
通讯作者：
P. Sexton;C. J. Gerard
影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
14.9
作者：
Sonnhammer EL;Östlund G
通讯作者：
Östlund G