Methods to collect Anopheles mosquitoes and evaluate malaria transmission: a comparative study in two villages in Senegal.
Methods to collect Anopheles mosquitoes and evaluate malaria transmission: a comparative study in two villages in Senegal.
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DOI：
10.1186/1475-2875-10-270
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发表时间：
2011-09-19
期刊：
影响因子：
3
通讯作者：
Trape JF
中科院分区：
文献类型：
--
作者：
Ndiath MO;Mazenot C;Gaye A;Konate L;Bouganali C;Faye O;Sokhna C;Trape JF
在疟疾传播的昆虫学研究中，人们研究了各种方法来代替人类着陆捕获（HLC）进行蚊子取样。在比较不同方法的相对效率时，根据面积、存在的物种及其密度，得到了相互矛盾的结果。这项研究的目的是通过三种不同的方法：高效液相色谱法、靠近蚊帐的灯诱法（LT/N）、除虫菊喷雾捕蚊法（PSC），比较塞内加尔两个地区取样的蚊子数量和特征。收集工作在Dielmo（苏丹稀树草原）和Bandafassi（苏丹几内亚稀树草原）两个村庄进行，在2001-2002年最大传播季节的4-5个月期间，每月进行两到三个晚上。采用酶联免疫吸附法（ELISA）鉴定疟原虫种类和感染情况。计算了具体组成、环孢子子蛋白率和昆虫学接种率。LT/N法捕获蚊种多样性最大，PSC法捕获蚊种多样性最小。每晚捕获按蚊的平均数量根据所采用的方法和种类有显著差异。PSC按蚊密度明显降低。以冈比亚按蚊为主要媒介（班达法西）时，高效液相色谱法是最有效的取样方法；LT/N时为funestus按蚊（Dielmo）。hplc与LT/M呈极显著相关，但相关参数因种而异。环孢子子蛋白率在不同方法和物种间无显著差异。昆虫接种率随病媒密度、方法和种类的不同而不同。采样方法的选择影响昆虫学数据的记录。因此，采样技术必须根据研究的载体和研究的目的来选择。只有hplc必须作为参考方法，但在某些情况下，LT/N可以作为替代方法。
Various methods have been studied as replacement of human landing catches (HLC) for mosquito sampling in entomological studies on malaria transmission. Conflicting results have been obtained in comparing relative efficiency of alternative methods, according to the area, the species present and their density. The aim of this study was to compare the number and characteristics of mosquitoes sampled in two areas of Senegal by three different methods: HLC, light traps adjacent to an occupied bed net (LT/N), pyrethrum spray catches (PSC). Collections were performed in two villages: Dielmo (Soudan savanna) and Bandafassi (Soudan Guinean savanna), two or three nights per month for a 4-5 months period during the maximal transmission season in 2001-2002. Species were identified and Plasmodium infection determined by ELISA. The specific composition, circumsporozoite protein rate and entomological inoculation rate were calculated. The diversity of mosquito species captured was maximal with LT/N, minimal with PSC. The mean number of anopheles captures each night was significantly different according to the method used and the species. PSC displayed a significantly lower anopheles density. HLC was the most efficient sampling method when Anopheles gambiae was the main vector (in Bandafassi); LT/N when it was Anopheles funestus (in Dielmo). A significant correlation was found between HLC and LT/M but correlation parameters were different according to the species. Circumsporozoite protein rates were not significantly different between methods or species. The entomological inoculation rate varied along with vector density and thus with methods and species. The choice of sampling method influenced entomological data recorded. Therefore, the sampling technique has to be chosen according to the vector studied and the aim of the study. Only HLC must be considered as the reference method, but in some conditions LT/N can be used as an alternative method.
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影响因子：
1.9
作者：
DAVIS, JR;HALL, T;SHIFF, CJ
通讯作者：
SHIFF, CJ
影响因子：
1.9
作者：
GITHEKO, AK;SERVICE, MW;JUMA, FO
通讯作者：
JUMA, FO
影响因子：
3
作者：
Mathenge EM;Misiani GO;Oulo DO;Irungu LW;Ndegwa PN;Smith TA;Killeen GF;Knols BG
通讯作者：
Knols BG
DOI：
10.4269/ajtmh.1997.56.247
发表时间：
1997-03-01
影响因子：
3.3
作者：
Fontenille, D;Lochouarn, L;Trape, JF
通讯作者：
Trape, JF
影响因子：
1.9
作者：
LINES, JD;CURTIS, CF;NJUNWA, KJ
通讯作者：
NJUNWA, KJ