First report of anthracnose caused by Colletotrichum fructicola on Brassica parachinensis in China
First report of anthracnose caused by Colletotrichum fructicola on Brassica parachinensis in China
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我国首次报道白菜炭疽病炭疽病
DOI：
10.1016/j.cropro.2021.105842
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发表时间：
2021-10
期刊：
影响因子：
2.8
通讯作者：
Zifu He
中科院分区：
文献类型：
--
作者：
Lin Yu;Guobing Lan;Yuguo Yang;Yafei Tang;Zhenggang Li;Xiaoman She;Zifu He
炭疽病是菜心上的一种毁灭性病害，严重影响菜心的生产，造成严重的经济损失。2012年至2018年期间，对B.表现出炭疽病样症状，即不规则和轻微凹陷。在开阔的田野中收集具有或不具有小黑点症状的灰色至棕色的病变。中国南方广东省11个地级市的菜心（parachinensis）。从单孢培养中获得102个炭疽菌分离物。根据培养特征和形态特征，分别鉴定出86个和13个菌株为C. higginsianum和C. truncatum，分别。而其它3个菌株在形态上与C. higginsianum和C. truncatum，但与C. fructicola..为了确认鉴定，扩增了内转录间隔区（ITS）、肌动蛋白（ACT）、几丁质合成酶1（CHS 1）、甘油醛-3-磷酸脱氢酶（GAPDH）和β-微管蛋白（TUB 2）基因。系统进化分析表明，这3个分离物的ITS、ACT、CHS 1、GAPDH和TUB 2序列均与原型菌株E18581的序列聚在一起。后验概率值为1..致病性测定表明，这3个菌株均能侵染健康的叶、茎和花。parachinensis并引起最初观察到的典型叶和茎腐烂症状，也会引起花腐烂症状。这在B上很少发生。parachinensis在田野里。从接种的植物的病斑中再次分离到相同的病原体。分布调查表明，C. Higginsianum分布广泛，是引起B炭疽病的主要病原菌。广东产的拟山慈菇。C. fructicola和C.元宝枫在广东省的分布范围为南北向和东西向。据我们所知，这是C.果生菌作为B炭疽病的病原。parachinensis在中国。
Anthracnose caused by Colletotrichum species is a devastating disease on Brassica parachinensis which affect the.crop production and cause economic losses in China. During the period from 2012 through 2018, samples of.diseased leaves of B. parachinensis showing anthracnose-like symptoms, that is irregular and slightly sunken.lesions in gray to brown color with or without small black spots symptoms were collected in open fields of.B. parachinensis from 11 prefecture-level cities of Guangdong province in southern China. One hundred and two.isolates of Colletotrichum were obtained from single spore cultures. Based on cultural and morphological char-.acteristics, 86 and 13 isolates were identified as C. higginsianum and C. truncatum, respectively. However, other.three isolates were morphologically different from C. higginsianum and C. truncatum, but similar to C. fructicola..To confirm the identification, the internal transcribed spacer (ITS) region, actin (ACT), chitin synthase 1 (CHS1),.glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and beta-tubulin (TUB2) genes were amplified. Phyloge-.netic analysis showed that the ITS, ACT, CHS1, GAPDH and TUB2 sequences of these three isolates clustered with.sequences belonging to the ex-type strain ICMP 18581 of C. fructicola with a posterior probability value of 1..Pathogenicity assays showed that all these three isolates could infect healthy leaves, stems and flowers of.B. parachinensis and cause typical leaf and stem rot symptoms originally observed and also flower rot symptoms.that rarely occurred on B. parachinensis in the fields. The same pathogen was re-isolated from lesions of inoc-.ulated plants. Distribution investigation showed that C. higginsianum is widely distributed and is the major.pathogen causing anthracnose of B. parachinensis in Guangdong. C. fructicola and C. truncatum distributed across.through the north to the south regions and through the east to the west regions of Guangdong, respectively. To.our knowledge, this is the first report of C. fructicola as a causal agent of anthracnose on B. parachinensis in China.
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DOI：
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发表时间：
2009
期刊：
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影响因子：
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作者：
通讯作者：
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