Human circulating fibrocytes have the capacity to differentiate osteoblasts and chondrocytes.
Human circulating fibrocytes have the capacity to differentiate osteoblasts and chondrocytes.
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DOI：
10.1016/j.biocel.2009.12.011
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发表时间：
2010-05
影响因子：
4
通讯作者：
Strieter, Robert M.
中科院分区：
文献类型：
--
作者：
Choi, Young H.;Burdick, Marie D.;Strieter, Robert M.
纤维细胞是骨髓源性细胞。纤维细胞可以分化为脂肪细胞和肌成纤维细胞样细胞。由于纤维细胞的行为类似于间充质祖细胞，我们假设纤维细胞有分化为其他间充质系细胞的潜力，如成骨细胞和软骨细胞。在本研究中，我们发现成纤维细胞在成骨介质中以类似于成骨细胞前体细胞的方式分化为成骨细胞样细胞。在这些条件下，纤维细胞和成骨细胞前体细胞表现出钙沉积增加，特定成骨基因表达增加。此外，在这些条件下，cAMP反应元件结合蛋白的去磷酸化与NF-κB受体激活剂配基/护骨素基因表达的增加和Osterix基因表达的增强有关。这两个事件在促进成骨方面都很重要。相反，在含有转化生长因子-β-3的软骨细胞培养液中培养的纤维细胞和间充质干细胞可分化为软骨细胞样细胞。在这些条件下的纤维细胞和间充质干细胞被发现表达增加的聚集素和II型胶原基因。在这些条件下，与软骨形成相关的转录因子基因也被发现在纤维细胞和间充质干细胞中被诱导。相反，在这些条件下，β-连环蛋白和核心结合因子α1亚单位蛋白转录因子的表达降低。这些数据表明，人纤维细胞具有分化为成骨细胞和软骨细胞样细胞的能力。这些发现表明，这种细胞可以用于基于细胞的组织再生治疗。
Fibrocytes are bone marrow-derived cells. Fibrocytes can differentiate into adipocyte- and myofibroblast-like cells. Since fibrocytes can behave like mesenchymal progenitor cells, we hypothesized that fibrocytes have the potential to differentiate into other mesenchymal lineage cells, such as osteoblasts and chondrocytes. In this study, we found that fibrocytes differentiated into osteoblast-like cells when cultured in osteogenic media in a manner similar to osteoblast precursor cells. Under these conditions, fibrocytes and osteoblast precursor cells displayed increased calcium deposition, and increased expression of specific osteogenic genes. In addition, dephosphorylation of cAMP-responsive element binding protein was associated with the increased ratio of Receptor activator of the NF-κB Ligand/osteoprotegerin gene expression and enhanced gene expression of osterix in these cells under these conditions. Both events are important in promoting osteogenesis. In contrast, fibrocytes and mesenchymal stem cells cultured in chondrogenic media in the presence of transforming growth factor-β3 were found to differentiate to chondrocyte-like cells. Fibrocytes and mesenchymal stem cells under these conditions were found to express increased levels of aggrecan and type II collagen genes. Transcription factor genes associated with chondrogenesis were also found to be induced in fibrocytes and mesenchymal stem cells under these conditions. In contrast, β–catenin protein and the core binding factor alpha1 subunit protein transcription factor were decreased in expression under these conditions. These data indicate that human fibrocytes have the capability to differentiate into osteoblast- and chondrocyte-like cells. These findings suggest that such cells could be used in cell-based tissue-regenerative therapy.
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DOI：
10.1073/pnas.94.12.6307
发表时间：
1997-06-10
影响因子：
11.1
作者：
Chesney, J;Bacher, M;Bucala, R
通讯作者：
Bucala, R
影响因子：
4.8
作者：
Hong, Kurt M.;Belperio, John A.;Strieter, Robert M.
通讯作者：
Strieter, Robert M.
影响因子：
4.2
作者：
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通讯作者：
Boskey, A
影响因子：
7
作者：
Lefebvre, V;Behringer, RR;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
DOI：
10.1016/j.biocel.2008.02.012
发表时间：
2008-01-01
影响因子：
4
作者：
Andersson-Sjoland, Annika;de Alba, Carolina Garcia;Selman, Moises
通讯作者：
Selman, Moises