Characterization of Deltacoronavirus in Black-Headed Gulls (Chroicocephalus ridibundus) in South China Indicating Frequent Interspecies Transmission of the Virus in Birds.
Characterization of Deltacoronavirus in Black-Headed Gulls (Chroicocephalus ridibundus) in South China Indicating Frequent Interspecies Transmission of the Virus in Birds.
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华南黑头鸥 (Chroicocephalusridibundus) 中 Delta 冠状病毒的特征表明该病毒在鸟类中频繁发生种间传播
DOI：
10.3389/fmicb.2022.895741
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
三角洲冠状病毒（DCoV）是常见于禽类和猪中的冠状病毒（CoV）属，但一些 DCoV 能够感染人类，这引起了人们对 DCoV 种间传播的担忧。因此，监测社区附近动物中 DCoV 的存在对于流行病预防具有重要意义。黑头鸥（Chroicocephalus rodibundus）是常见的候鸟，栖息于中国云南省大部分城乡湿地，是黑头鸥越冬的典型栖息地。云南红嘴鸥是否携带冠状病毒尚未确定。在这项研究中，我们通过逆转录PCR鉴定了云南黑头鸥粪便样本中的三种DCoV病毒株，并对它们的全基因组进行了测序。基因组分析显示，这三个毒株的基因组同一性超过99%，因此命名为DCoV HNU4-1、HNU4-2和HNU4-3；它们的NSP12与猎鹰冠状病毒UAE-HKU27（HKU27）、Houbara冠状病毒UAE-HKU28（HKU28）和鸽子冠状病毒UAE-HKU29（HKU29）的同源物具有高度相似性。由于HKU28和HKU29均在迪拜发现，因此这些禽类DCoV可能通过特定途径跨境传播。进一步的共同进化分析支持了这一推测，即黑头鸥中的 HNU4（或其祖先）起源于凤原鸥中的 HKU28（或其同源品系），这是两个不同鸟类目之间的种间传播。此外，还预测了 DCoV 的种间传播，从凤原到猎鹰、鸽子和绣眼鸟，从麻雀到喜鹊，以及鹌鹑和包括猪和亚洲豹猫在内的哺乳动物，从文鸟到喜鹊。这是亚洲首次报道黑头鸥DCoV，与其他禽类DCoV高度同源，预测了DCoV非常“活跃”的宿主转换事件，为研究DCoV的传播和传播提供了重要参考。
Deltacoronavirus (DCoV) is a genus of coronavirus (CoV) commonly found in avian and swine, but some DCoVs are capable of infecting humans, which causes the concern about interspecies transmission of DCoVs. Thus, monitoring the existence of DCoVs in animals near communities is of great importance for epidemic prevention. Black-headed gulls (Chroicocephalus ridibundus) are common migratory birds inhabiting in most urban and rural wetlands of Yunnan Province, China, which is a typical habitat for black-headed gulls to overwinter. Whether Yunnan black-headed gulls carry CoV has never been determined. In this study, we identified three strains of DCoVs in fecal samples of Yunnan black-headed gulls by reverse-transcriptional PCR and sequenced their whole genomes. Genomic analysis revealed that these three strains shared genomic identity of more than 99%, thus named DCoV HNU4-1, HNU4-2, and HNU4-3; their NSP12 showed high similarity of amino acid sequence to the homologs of falcon coronavirus UAE-HKU27 (HKU27), houbara coronavirus UAE-HKU28 (HKU28), and pigeon coronavirus UAE-HKU29 (HKU29). Since both HKU28 and HKU29 were found in Dubai, there might be cross-border transmission of these avian DCoVs through specific routes. Further coevolutionary analysis supported this speculation that HNU4 (or its ancestors) in black-headed gulls originated from HKU28 (or its homologous strain) in houbara, which was interspecies transmission between two different avian orders. In addition, interspecies transmission of DCoV, from houbara to falcon, pigeon and white-eye, from sparrow to common-magpie, and quail and mammal including porcine and Asian leopard cat, from munia to magpie-robin, was predicted. This is the first report of black-headed gull DCoV in Asia which was highly homolog to other avian DCoVs, and the very “active” host-switching events in DCoV were predicted, which provides important reference for the study of spread and transmission of DCoVs.
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影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
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影响因子：
5.4
作者：
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影响因子：
10.7
作者：
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Minh BQ
影响因子：
5
作者：
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影响因子：
6.4
作者：
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通讯作者：
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