Respiratory and Intramuscular Immunization With ChAdOx2-NPM1-NA Induces Distinct Immune Responses in H1N1pdm09 Pre-Exposed Pigs.
Respiratory and Intramuscular Immunization With ChAdOx2-NPM1-NA Induces Distinct Immune Responses in H1N1pdm09 Pre-Exposed Pigs.
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DOI：
10.3389/fimmu.2021.763912
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发表时间：
2021
影响因子：
7.3
通讯作者：
Tchilian E
中科院分区：
文献类型：
--
作者：
Vatzia E;Allen ER;Manjegowda T;Morris S;McNee A;Martini V;Kaliath R;Ulaszewska M;Boyd A;Paudyal B;Carr VB;Chrun T;Maze E;MacLoughlin R;van Diemen PM;Everett HE;Lambe T;Gilbert SC;Tchilian E
迫切需要开发提供更广泛保护的上级流感疫苗。流感疫苗传统上在未感染的动物中进行测试，尽管人类在生命的最初几年中暴露于流感，但先前的流感暴露对疫苗免疫应答的影响尚未得到充分研究。猪是流感的重要天然宿主，是大流行性病毒的来源，并且是人类流感的极好模型。在这里，我们研究了ChAdOx 2病毒载体疫苗的免疫原性，表达流感核蛋白，基质蛋白1，和神经氨酸酶在H1N1pdm09预暴露的猪。我们通过比较鼻内、气雾剂和肌内免疫来评价给药途径的重要性。气雾剂递送促进了局部肺T细胞和抗体应答，而肌肉注射免疫促进了外周血免疫。这些结果将为如何最好地提供疫苗以利用最佳保护性免疫提供信息。
There is a critical need to develop superior influenza vaccines that provide broader protection. Influenza vaccines are traditionally tested in naive animals, although humans are exposed to influenza in the first years of their lives, but the impact of prior influenza exposure on vaccine immune responses has not been well studied. Pigs are an important natural host for influenza, are a source of pandemic viruses, and are an excellent model for human influenza. Here, we investigated the immunogenicity of the ChAdOx2 viral vectored vaccine, expressing influenza nucleoprotein, matrix protein 1, and neuraminidase in H1N1pdm09 pre-exposed pigs. We evaluated the importance of the route of administration by comparing intranasal, aerosol, and intramuscular immunizations. Aerosol delivery boosted the local lung T-cell and antibody responses, while intramuscular immunization boosted peripheral blood immunity. These results will inform how best to deliver vaccines in order to harness optimal protective immunity.
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影响因子：
4.4
作者：
Holzer, Barbara;Morgan, Sophie B.;Tchilian, Elma
通讯作者：
Tchilian, Elma
影响因子：
32.4
作者：
Jarjour NN;Masopust D;Jameson SC
通讯作者：
Jameson SC
影响因子：
7.3
作者：
Edmans M;McNee A;Porter E;Vatzia E;Paudyal B;Martini V;Gubbins S;Francis O;Harley R;Thomas A;Burt R;Morgan S;Fuller A;Sewell A;Charleston B;Bailey M;Tchilian E
通讯作者：
Tchilian E
影响因子：
4.4
作者：
Hemmink JD;Morgan SB;Aramouni M;Everett H;Salguero FJ;Canini L;Porter E;Chase-Topping M;Beck K;Loughlin RM;Carr BV;Brown IH;Bailey M;Woolhouse M;Brookes SM;Charleston B;Tchilian E
通讯作者：
Tchilian E
DOI：
10.1164/rccm.201411-1988oc
发表时间：
2015-06-15
影响因子：
24.7
作者：
Hayward, Andrew C.;Wang, Lili;McMichael, Andrew J.
通讯作者：
McMichael, Andrew J.