Proteomics of the lysosome.
Proteomics of the lysosome.
复制标题
溶酶体的蛋白质组学。
DOI：
10.1016/j.bbamcr.2008.09.018
复制
发表时间：
2009-04
期刊：
影响因子：
--
通讯作者：
Sleat DE
中科院分区：
文献类型：
--
作者：
Lübke T;Lobel P;Sleat DE
溶酶体功能的缺陷与许多单基因人类疾病相关，这些疾病通常被归类为溶酶体贮积病。然而，越来越多的证据表明，溶酶体蛋白也参与了更广泛的人类疾病，包括癌症和阿尔茨海默病。因此，有一个持续的兴趣，了解细胞功能的溶酶体和一个新兴的方法，这是确定其组成蛋白质的蛋白质组学分析。迄今为止，哺乳动物溶酶体已被证明含有约60种可溶性腔蛋白和约25种跨膜蛋白。然而，最近的蛋白质组学研究的基础上亲和纯化的可溶性成分或亚细胞分级分离，以获得可溶性和膜成分表明，可能有更多的这两类蛋白质居民在这个细胞器比以前认识到的。这些蛋白质的发现对于理解溶酶体的功能和动力学具有重要意义，但也可以导致发现迄今未知病因的人类疾病的遗传基础。在这里，我们描述了目前的方法，溶酶体蛋白质组学和数据解释和审查新的溶酶体蛋白，最近出现的研究。
Defects in lysosomal function have been associated with numerous monogenic human diseases typically classified as lysosomal storage diseases. However, there is increasing evidence that lysosomal proteins are also involved in more widespread human diseases including cancer and Alzheimer disease. Thus, there is a continuing interest in understanding the cellular functions of the lysosome and an emerging approach to this is the identification of its constituent proteins by proteomic analyses. To date, the mammalian lysosome has been shown to contain ~ 60 soluble luminal proteins and ~25 transmembrane proteins. However, recent proteomic studies based upon affinity purification of soluble components or subcellular fractionation to obtain both soluble and membrane components suggest that there may be many more of both classes of protein resident within this organelle than previously appreciated. Discovery of such proteins has important implications for understanding the function and the dynamics of the lysosome but can also lead the way towards the discovery of the genetic basis for human diseases of hitherto unknown etiology. Here, we describe current approaches to lysosomal proteomics and data interpretation and review the new lysosomal proteins that have recently emerged from such studies.
登录
查看更多内容
影响因子：
2.7
作者：
ARAI, K;KANASEKI, T;OHKUMA, S
通讯作者：
OHKUMA, S
影响因子：
7
作者：
Czupalla, C;Mansukoski, H;Hoflack, B
通讯作者：
Hoflack, B
影响因子：
3.5
作者：
ARBORGH, B;ERICSSON, JL;GLAUMANN, H
通讯作者：
GLAUMANN, H
影响因子：
64.5
作者：
Foster, LJ;de Hoog, CL;Mann, M
通讯作者：
Mann, M
影响因子：
4.8
作者：
Frese, Marc-Andre;Schulz, Stefanie;Dierks, Thomas
通讯作者：
Dierks, Thomas