Hyphal orientation of Candida albicans is regulated by a calcium-dependent mechanism.
Hyphal orientation of Candida albicans is regulated by a calcium-dependent mechanism.
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白色念珠菌的菌丝取向受钙依赖性机制调节。
DOI：
10.1016/j.cub.2006.12.043
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发表时间：
2007-02-20
期刊：
影响因子：
9.2
通讯作者：
Gow, Neil A. R.
中科院分区：
文献类型：
--
作者：
Brand, Alexandra;Shanks, Scott;Duncan, Vanessa M. S.;Yang, Meng;Mackenzie, Kevin;Gow, Neil A. R.
从真菌菌丝到神经突的真核细胞，通过极化延伸生长，必须协调细胞生长和细胞定向，使它们能够表现出生长向性并对相关的环境信号作出反应。这些细胞通常保持尖端高Ca 2+细胞质梯度，这与它们表现出极化尖端生长和响应生长导向细胞外信号的能力相关。在酵母和其他真菌中，polarisome，exocyst，Arp 2/3和Spitzenkörper蛋白复合物共同协调尖端生长和细胞极性，但目前尚不清楚这些分子复合物是否也调节细胞取向或它们是否受到细胞质Ca 2+梯度的影响。人类致病真菌白色念珠菌的菌丝重新定向其生长轴，以响应底层表面形貌（向触性）和施加的电场（向电流性）。定向生长的建立和维持与这些环境线索是Ca 2+依赖的。在含有低Ca 2+或钙通道阻滞剂的培养基中，以及在钙通道或钙信号传导途径的元素被删除的突变体中，向性减弱。因此，向电流性和向触性都可能是通过适当的环境信号激活的Ca 2+通道在极化生长部位的局部Ca 2+内流介导的。
Eukaryotic cells from fungal hyphae to neurites that grow by polarized extension must coordinate cell growth and cell orientation to enable them to exhibit growth tropisms and to respond to relevant environmental cues. Such cells generally maintain a tip-high Ca2+ cytoplasmic gradient, which is correlated with their ability to exhibit polarized tip growth and to respond to growth-directing extracellular signals. In yeast and other fungi, the polarisome, exocyst, Arp2/3, and Spitzenkörper protein complexes collectively orchestrate tip growth and cell polarity, but it is not clear whether these molecular complexes also regulate cell orientation or whether they are influenced by cytoplasmic Ca2+ gradients. Hyphae of the human pathogenic fungus Candida albicans reorient their growth axis in response to underlying surface topography (thigmotropism) and imposed electric fields (galvanotropism). The establishment and maintenance of directional growth in relation to these environmental cues was Ca2+ dependent. Tropisms were attenuated in media containing low Ca2+, or calcium-channel blockers, and in mutants where calcium channels or elements of the calcium signaling pathway were deleted. Therefore galvanotropism and thigmotropism may both be mediated by localized Ca2+ influx at sites of polarized growth via Ca2+ channels that are activated by appropriate environmental signals.
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DOI：
10.1073/pnas.0501961102
发表时间：
2005-07-26
影响因子：
11.1
作者：
Beaumont, V;Llobet, A;Lagnado, L
通讯作者：
Lagnado, L
影响因子：
5.3
作者：
IIDA, H;NAKAMURA, H;ANRAKU, Y
通讯作者：
ANRAKU, Y
影响因子：
5.3
作者：
Paidhungat, M;Garrett, S
通讯作者：
Garrett, S
影响因子：
3.7
作者：
Ozeki-Miyawaki, C;Moriya, Y;Sokabe, M
通讯作者：
Sokabe, M
影响因子：
5.3
作者：
Locke, EG;Bonilla, M;Cunningham, KW
通讯作者：
Cunningham, KW