Viral messenger RNAs in six lines of adenovirus-transformed cells.
Viral messenger RNAs in six lines of adenovirus-transformed cells.
复制标题
六系腺病毒转化细胞中的病毒信使 RNA。
DOI：
10.1016/0042-6822(81)90116-1
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发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
M. Mathews
中科院分区：
文献类型：
--
作者：
J. B. Lewis;M. Mathews
我们比较了在体外使用来自六个腺病毒转化细胞系（F4、F17、F19、REM、T2 C4和293）的病毒特异性mRNA的蛋白质与使用来自生产性感染细胞的RNA的蛋白质。在所有细胞系中检测到蛋白质E1 A-58 K、54 K、48 K和42 K以及E1 B-15 K的mRNA，并且在至少五个细胞系中检测到E1 B-57 K的mRNA。在特定条件下在感染细胞中发现的次要E1 A mRNA（蛋白质25 K和28 K）在任何转化细胞系中均未发现。两个细胞系，F4和T2 C4，含有少量的其他mRNA编码的区域E1 B内，包括在后一种情况下，一个没有检测到感染的细胞。系F4、REM和T2 C4含有至少一些E4 mRNA，在后一种情况下，还包括在感染细胞中未检测到的E4 mRNA。T2 C4细胞还含有E3 mRNA、E2 A-72 K蛋白mRNA和两种E2 B mRNA，尽管E2 B蛋白与感染细胞中发现的任何蛋白都不相同。F19和T2 C4系表达编码11至17个图谱单位的mRNA，即“IVa 2区”，但不包括多肽IVa 2本身的mRNA。一些早期mRNA，包括“立即早期”mRNA，在任何这些转化细胞系中均未检测到：细胞系也不含有仅在生产性感染后期发现的任何mRNA。这些结果与细胞特性的比较初步表明，额外病毒基因的表达与致瘤性增加之间存在相关性（尽管不一定是因果关系）。
We have compared the proteins translatedin vitrousing virus-specific mRNA from six lines of adenovirus-transformed cells (F4, F17, F19, REM, T2C4, and 293) with those made using RNA from productively infected cells. The mRNAs for proteins E1A-58K, 54K, 48K, and 42K, and for E1B-15K, were detected in all cell lines and for E1B-57K in at least five of the lines. Neither of the minor E1A mRNAs (for proteins 25K and 28K) found under specific conditions in infected cells were found in any transformed cell line. Two cell lines, F4 and T2C4, contained small amounts of other mRNAs encoded within region E1B including in the latter case one not detected in infected cells. The lines F4, REM, and T2C4 contain at least some E4 mRNAs, again including in the latter case one not detected in infected cells. T2C4 cells also contain E3 mRNAs, E2A-72K protein mRNA, and two E2B mRNAs, although the E2B proteins are not identical to any found in infected cells. The F19 and T2C4 lines express mRNAs encoded between 11 and 17 map units, the “IVa2region,” but do not include the mRNA for polypeptide IVa2itself. Some early mRNAs, including the “immediate early” mRNAs, have not been detected in any of these lines of transformed cells: nor did the cell lines contain any mRNAs found exclusively at late times during productive infection. Comparison of these results with the properties of the cells tentatively suggest a correlation (although not necessarily causal) between the expression of additional viral genes and increased tumorigenicity.
DOI：
10.1101/sqb.1980.044.01.044
发表时间：
1979-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
CHOW, LT;LEWIS, JB;BROKER, TR
通讯作者：
BROKER, TR
腺病毒2号主要晚期转录单位中信使RNA和蛋白质编码序列的排列。
影响因子：
5.6
作者：
Miller,JS;Ricciardi,RP;Roberts,BE;Paterson,BM;Mathews,MB
通讯作者：
Mathews,MB