Arrangement of messenger RNAs and protein coding sequences in the major late transcription unit of adenovirus 2.
Arrangement of messenger RNAs and protein coding sequences in the major late transcription unit of adenovirus 2.
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腺病毒2号主要晚期转录单位中信使RNA和蛋白质编码序列的排列。
DOI：
10.1016/0022-2836(80)90258-2
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发表时间：
1980
影响因子：
5.6
通讯作者：
Mathews,MB
中科院分区：
文献类型：
--
作者：
Miller,JS;Ricciardi,RP;Roberts,BE;Paterson,BM;Mathews,MB
利用三种互补的分析方法表征了腺病毒2感染晚期HeLa细胞中的信使rna。在每种情况下，mrna都是通过识别它们在网状细胞无细胞系统中翻译时编码的多肽来定义功能的。通过(1)在含有甲基氢氧化汞的琼脂糖凝胶中电泳分离来估计功能rna的大小；(2)对固定在硝化纤维素上的病毒DNA片段进行退火选择，确定其组成序列的基因组起源；(3)利用病毒DNA片段进行杂交阻滞翻译，定位其蛋白质编码序列。该结果允许所有晚期mrna的编码区和非编码区相对于DNA的定位。他们证明起源于主要晚期转录单位的成熟mrna分为五个家族。在每个家族中，mrna以交错的方式重叠，共享相同的3 ‘端，但在5 ’端不同。数据还揭示了存在的52000年和55000年的两个新的蛋白质末mrencoded地图坐标30到34岁之间和允许晚期rna的定位,根据他们编码的多肽,如下:52岁,55 K·III a: III·pVII·V:元太·II: 100 K / 33 K·pVIII:第四。(冒号表示结束的家庭,家庭内部中心圆点连接;削减重叠的编码序列;逗号,编码序列,没有分离分析。55k蛋白质的mr = 55000。)在含有甲基氢氧化汞的琼脂糖凝胶中，那些要么是来自特定区域的唯一转录本（如多肽IX， IVa2和IV的转录本），要么是家族中3 '端转录本（如编码多肽IIIa， V， II和pVIII的转录本）的mrna在电泳时作为离散物种迁移。然而，一个家族内的5 ' -近端rna（如编码多肽52,55k， III, pVII, pVI， 100k和33k的rna）表现出广泛的分子量分布。
The messenger RNAs present in HeLa cells late in adenovirus 2 infection have been characterized utilizing three complementary methods of analysis. In each case the mRNAs were defined functionally, by identification of the polypeptides they encode when translated in a reticulocyte cell-free system. The RNAs were examined by (1) electrophoretic fractionation in agarose gels containing methyl-mercury hydroxide to estimate the sizes of the functional RNAs; (2) selection by annealing to viral DNA fragments immobilized on nitrocellulose to define the genomic origin of their constituent sequences; (3) hybrid-arrested translation using viral DNA fragments to locate their protein coding sequence.The results allow the positioning of both the coding and non-coding regions of all the late mRNAs with respect to the DNA. They demonstrate that the mature mRNAs originating in the major late transcription unit fall into five families. Within each family the mRNAs overlap in a staggered fashion, sharing the same 3′ terminus but differing at their 5′ termini. The data also reveal the existence of two new late proteins of 52,000 and 55,000Mrencoded between map co-ordinates 30 to 34 and permit the positioning of the late RNAs, according to the polypeptides they encode, as follows: 52, 55K · IIIa: III · pVII · V: pVI · II: 100 K/33 K · pVIII: IV.(The colons signify the ends of families; centre dots the junctions within families; the slash an overlap of coding sequences; and the comma, coding sequences which have not been separated in this analysis. A 55 K protein hasMr= 55,000.)Those mRNAs which are either the only transcripts from a specific region (such as those for polypeptides IX, IVa2 and IV) or are the 3′-terminal transcripts within a family (such as those coding for polypeptides IIIa, V, II and pVIII) migrate as discrete species upon electrophoresis in agarose gels containing methyl-mercury hydroxide. However, the 5′-proximal RNAs within a family (such as those encoding polypeptides 52, 55 K, III, pVII, pVI, 100 K and 33 K) exhibit a broad distribution of molecular weights.
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影响因子：
3.7
作者：
G. Akusjärvi;U. Pettersson
通讯作者：
U. Pettersson
影响因子：
64.5
作者：
DUNN, AR;HASSELL, JA
通讯作者：
HASSELL, JA
腺病毒多肽IX的基因和信使RNA
影响因子：
64.5
作者：
U. Pettersson;M. Mathews
通讯作者：
M. Mathews
2 型腺病毒基因组上早期和晚期信使 RNA 序列的杂交图谱。
影响因子：
5.6
作者：
U. Pettersson;C. Tibbetts;L. Philipson
通讯作者：
L. Philipson
影响因子：
5.6
作者：
BERGET, SM;SHARP, PA
通讯作者：
SHARP, PA