Trajectory of isoelectric focusing from gels to capillaries to immobilized gradients in capillaries.
Trajectory of isoelectric focusing from gels to capillaries to immobilized gradients in capillaries.
复制标题
DOI：
10.1002/pmic.201200213
复制
发表时间：
2012-10
期刊：
影响因子：
3.4
通讯作者：
Wirth, Mary J.
中科院分区：
文献类型：
--
作者：
Koshel, Brooke M.;Wirth, Mary J.
本文综述了在蛋白质组学的2D分离中取代凝胶的需要，其中速度，高通量和表征痕量水平蛋白质或小样品的能力是当前的需求。该综述的主题是等电聚焦，这是一个有价值的工具，因为它预浓缩蛋白质，除了分离高峰容量。本文回顾了从凝胶等电聚焦到cIEF再到固定化梯度填充毛细管等电聚焦的技术进展。多毛细管技术正朝着满足当前需求的方向发展，提供关于浓度和小样品的极高灵敏度、集成自动化以及来自多个分离维度的高峰容量。用于cIEF的具有固定pH梯度的Captain正在出现，这将减轻两性电解质的干扰，并提高分离时间的重现性，当这种有价值的技术可以用作维度之一时。
This review presents the need for replacing gels in 2D separations for proteomics, where speed, high-throughput, and the ability to characterize trace level proteins or small samples are the current desires. The theme of the review is isoelectric focusing, which is a valuable tool because it pre-concentrates proteins in addition to separating with high peak capacity. The review traces the technological progress from gel IEF to cIEF to packed capillaries with immobilized gradients for cIEF. Multiple capillary techniques are progressing toward meeting the current desires, providing extremely high sensitivity with regard to concentration and to small samples, integrated automation, and high peak capacity from multiple dimensions of separation. Capillaries with immobilized pH gradients for cIEF are emerging, which will alleviate interference from ampholytes and improve reproducibility in separation times when this valuable technique can be used as one of the dimensions.
登录
查看更多内容
影响因子：
7.4
作者：
Hua, Yimin;Koshel, Brooke M.;Wirth, Mary J.
通讯作者：
Wirth, Mary J.
影响因子：
7.4
作者：
Ramsay, Lauren M.;Dickerson, Jane A.;Dada, Oluwatosin;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.
影响因子：
2.9
作者：
Doherty, EAS;Meagher, RJ;Barron, AE
通讯作者：
Barron, AE
影响因子：
2.9
作者：
Liang, Yu;Zhu, Guijie;Zhang, Yukui
通讯作者：
Zhang, Yukui
影响因子：
4.1
作者：
Bo, T;Pawliszyn, J
通讯作者：
Pawliszyn, J