Alterations in biomechanical properties and microstructure of colon wall in early-stage experimental colitis.
Alterations in biomechanical properties and microstructure of colon wall in early-stage experimental colitis.
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早期实验性结肠炎中结肠壁生物力学特性和微观结构的变化
DOI：
10.3892/etm.2017.4607
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发表时间：
2017-08
影响因子：
2.7
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Gong X;Xu X;Lin S;Cheng Y;Tong J;Li Y
本研究旨在探讨葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎早期对结肠壁生物力学特性和微结构的影响。本研究用DSS灌胃诱导8周龄小鼠结肠炎模型，取10只正常对照和10只实验性结肠炎标本。进行单轴拉伸试验，测量结肠组织的极限拉伸强度和极限拉伸强度。此外，还进行了组织学研究，以表征治疗后结肠壁微结构的变化。结果显示，对照组和DSS组的极限拉应力分别为(232±33)和(183±25)kPa(P=0.001)。对照组和DSS组的断裂伸长值分别为1.43±0.04和1.51±0.06(P=0.006)。然而，两组之间在组织硬度方面没有统计学上的显著差异。组织学分析显示DSS组有大量炎性细胞渗入间质，导致明显的黏膜下水肿。在DSS处理的粘膜下层也发现了增生，导致结肠壁内微结构紊乱。此外，与对照组相比，DSS处理组肌层胶原纤维大量断裂，纤维束变细。总之，早期实验性结肠炎改变了结肠壁的机械性能和微观结构特征，进一步促进了病理过程中的组织重塑。
The aim of the current study was to investigate the effects of early-stage dextran sodium sulfate (DSS)-induced mouse colitis on the biomechanical properties and microstructure of colon walls. In the present study, colitis was induced in 8-week-old mice by the oral administration of DSS, and then 10 control and 10 experimental colitis samples were harvested. Uniaxial tensile tests were performed to measure the ultimate tensile strength and ultimate stretches of colon tissues. In addition, histological investigations were performed to characterize changes in the microstructure of the colon wall following treatment. The results revealed that the ultimate tensile stresses were 232±33 and 183±25 kPa for the control and DSS groups, respectively (P=0.001). Ultimate stretches at rupture for the control and DSS groups were 1.43±0.04 and 1.51±0.06, respectively (P=0.006). However, there was no statistically significant difference in tissue stiffness between the two groups. Histological analysis demonstrated high numbers of inflammatory cells infiltrated into the stroma in the DSS group, leading to significant submucosa edema. Hyperplasia was also identified in the DSS-treated submucosa, causing a disorganized microstructure within the colon wall. Furthermore, a large number of collagen fibers in the DSS-treated muscular layer were disrupted, and fiber bundles were thinner when compared with the control group. In conclusion, early-stage experimental colitis alters the mechanical properties and microstructural characteristics of the colon walls, further contributing to tissue remodeling in the pathological process.
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