GMAP210 and IFT88 are present in the spermatid golgi apparatus and participate in the development of the acrosome-acroplaxome complex, head-tail coupling apparatus and tail.
GMAP210 and IFT88 are present in the spermatid golgi apparatus and participate in the development of the acrosome-acroplaxome complex, head-tail coupling apparatus and tail.
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DOI：
10.1002/dvdy.22563
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发表时间：
2011-03
影响因子：
2.5
通讯作者：
Rios, Rosa M.
中科院分区：
文献类型：
--
作者：
Kierszenbaum, Abraham L.;Rivkin, Eugene;Tres, Laura L.;Yoder, Bradley K.;Haycraft, Courtney J.;Bornens, Michel;Rios, Rosa M.
我们描述了高尔金 GMAP210 和鞭毛内蛋白 IFT88 在精子细胞高尔基体中的定位，以及这两种蛋白在顶体-顶体复合体、头尾耦合装置 (HTCA) 和精子细胞尾部发育中的参与。免疫细胞化学实验表明，GMAP210 在顺式高尔基体网络中占主导地位，而 IFT88 在反式高尔基体网络中占主导地位。两种蛋白沿着顶体膜、HTCA 和发育中的尾部共定位于顶体小泡中。 IFT88 持续存在于精子头部的顶体-顶体区域，而 GMAP210 在那里不再可见。 Ift88 小鼠突变体的精子细胞表现出异常的头部形状并且没有尾巴。 GMAP210 在顶体-顶体生物发生过程中在 Ift88 突变体中可见。然而，GMAP210 染色的囊泡、线粒体和外部致密纤维材料在 manchette 区域积聚，无法到达突变体的败育尾残端。在体外，用 Brefeldin-A 和诺考达唑破坏精细胞高尔基体和微管，可阻止 GMAP210 和 IFT88 染色的原顶体囊泡向顶体-顶体复合体的进展，但顶体中 F-肌动蛋白的分布不受影响。我们提供了第一个证据，证明 IFT88 存在于精子细胞的高尔基体中，微管相关的高尔基体 GMAP210 和 IFT88 参与顶体、HTCA 和尾部生物发生，并且货物的缺陷内运输会破坏精子细胞尾部的发育。
We describe the localization of the golgin GMAP210 and the intraflagellar protein IFT88 in the Golgi of spermatids and the participation of these two proteins in the development of the acrosome-acroplaxome complex, the head-tail coupling apparatus (HTCA) and the spermatid tail. Immunocytochemical experiments show that GMAP210 predominates in the cis-Golgi whereas IFT88 prevails in the trans-Golgi network. Both proteins co-localize in proacrosomal vesicles, along acrosome membranes, the HTCA and the developing tail. IFT88 persists in the acrosome-acroplaxome region of the sperm head whereas GMAP210 is not longer seen there. Spermatids of the Ift88 mouse mutant display abnormal head shaping and are tail-less. GMAP210 is visualized in the Ift88 mutant during acrosome-acroplaxome biogenesis. However, GMAP210–stained vesicles, mitochondria and outer dense fiber material build up in the manchette region and fail to reach the abortive tail stump in the mutant. In vitro disruption of the spermatid Golgi and microtubules with Brefeldin-A and nocodazole blocks the progression of GMAP210- and IFT88-stained proacrosomal vesicles to the acrosome-acroplaxome complex but F-actin distribution in the acroplaxome is not affected. We provide the first evidence that IFT88 is present in the Golgi of spermatids, that the microtubule-associated golgin GMAP210 and IFT88 participate in acrosome, HTCA and tail biogenesis, and that defective intramanchette transport of cargos disrupts spermatid tail development.
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DOI：
10.1083/jcb.145.1.83
发表时间：
1999-04-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Infante C;Ramos-Morales F;Fedriani C;Bornens M;Rios RM
通讯作者：
Rios RM
影响因子：
2.5
作者：
Kierszenbaum, AL
通讯作者：
Kierszenbaum, AL
影响因子：
2.1
作者：
Kim, Hon-Song;Takahashi, Mikiko;Ono, Yoshitaka
通讯作者：
Ono, Yoshitaka
影响因子：
2.7
作者：
Moreno, RD;Palomino, J;Schatten, G
通讯作者：
Schatten, G
影响因子：
2.5
作者：
Lehman, Jonathan M.;Michaud, Edward J.;Schoeb, Trenton R.;Aydin-Son, Yesim;Miller, Michael;Yoder, Bradley K.
通讯作者：
Yoder, Bradley K.