Decreased miR-26a expression correlates with the progression of podocyte injury in autoimmune glomerulonephritis.
Decreased miR-26a expression correlates with the progression of podocyte injury in autoimmune glomerulonephritis.
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DOI：
10.1371/journal.pone.0110383
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kon Y
中科院分区：
文献类型：
--
作者：
Ichii O;Otsuka-Kanazawa S;Horino T;Kimura J;Nakamura T;Matsumoto M;Toi M;Kon Y
MicroRNAs参与某些疾病的发病机制，并可作为生物标志物。我们分析了B6中肾小球microRNA的表达。MRLc1，作为自身免疫性肾小球肾炎的小鼠模型。我们发现miR-26a是正常C57BL/6肾小球中表达最丰富的microRNA，其在B6肾小球中的表达。MRLc1明显低于C57BL/6。在小鼠肾脏中，足细胞主要表达miR-26a，肾小球中B6表达miR-26a。MRLc1小鼠与尿白蛋白水平和足细胞特异性基因表达呈负相关。puromycin诱导的永活小鼠足细胞损伤降低了miR-26a的表达，扰乱了肌动蛋白细胞骨架，增加了含有miR-26a的外泌体的释放。虽然miR-26a的表达随着永生化小鼠足细胞的分化而增加，但沉默miR-26a会降低与足细胞分化和细胞骨架形成相关的基因的表达。特别是，波形蛋白和肌动蛋白水平明显降低。狼疮性肾炎和IgA肾病患者肾小球miR-26a水平明显低于健康对照组。B6。MRLc1和狼疮性肾炎患者尿外泌体中miR-26a水平显著高于各自健康对照组。这些数据表明，miR-26a调节足细胞分化和细胞骨架完整性，其在肾小球和尿液中的水平改变可能作为自身免疫性肾小球肾炎中足细胞损伤的标志。
MicroRNAs contribute to the pathogenesis of certain diseases and may serve as biomarkers. We analyzed glomerular microRNA expression in B6.MRLc1, which serve as a mouse model of autoimmune glomerulonephritis. We found that miR-26a was the most abundantly expressed microRNA in the glomerulus of normal C57BL/6 and that its glomerular expression in B6.MRLc1 was significantly lower than that in C57BL/6. In mouse kidneys, podocytes mainly expressed miR-26a, and glomerular miR-26a expression in B6.MRLc1 mice correlated negatively with the urinary albumin levels and podocyte-specific gene expression. Puromycin-induced injury of immortalized mouse podocytes decreased miR-26a expression, perturbed the actin cytoskeleton, and increased the release of exosomes containing miR-26a. Although miR-26a expression increased with differentiation of immortalized mouse podocytes, silencing miR-26a decreased the expression of genes associated with the podocyte differentiation and formation of the cytoskeleton. In particular, the levels of vimentin and actin significantly decreased. In patients with lupus nephritis and IgA nephropathy, glomerular miR-26a levels were significantly lower than those of healthy controls. In B6.MRLc1 and patients with lupus nephritis, miR-26a levels in urinary exosomes were significantly higher compared with those for the respective healthy control. These data indicate that miR-26a regulates podocyte differentiation and cytoskeletal integrity, and its altered levels in glomerulus and urine may serve as a marker of injured podocytes in autoimmune glomerulonephritis.
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影响因子：
4.2
作者：
Kajiyama H;Titus S;Austin CP;Chiotos K;Matsumoto T;Sakairi T;Kopp JB
通讯作者：
Kopp JB
影响因子：
18.2
作者：
Greka A;Mundel P
通讯作者：
Mundel P
影响因子：
19.6
作者：
Kim, JH;Ha, IS;Park, WY
通讯作者：
Park, WY
尿外泌体/微泡中的 MicroRNA-29c 作为肾纤维化的生物标志物
DOI：
10.1152/ajprenal.00148.2013
发表时间：
2013-10-01
影响因子：
4.2
作者：
Lv, Lin-Li;Cao, Yu-Han;Liu, Bi-Cheng
通讯作者：
Liu, Bi-Cheng
影响因子：
19.6
作者：
Alvarez, M. Lucrecia;Khosroheidari, Mahdieh;DiStefano, Johanna K.
通讯作者：
DiStefano, Johanna K.