LAP-MALDI MS Profiling and Identification of Potential Biomarkers for the Detection of Bovine Tuberculosis.
LAP-MALDI MS Profiling and Identification of Potential Biomarkers for the Detection of Bovine Tuberculosis.
复制标题
LAP-MALDI MS分析和潜在生物标志物检测牛结核病。
DOI：
10.1021/acs.jafc.3c01879
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发表时间：
2023-09-20
影响因子：
6.1
通讯作者：
Cramer, Rainer
中科院分区：
文献类型：
--
作者：
Lellman, Sophie E.;Reynolds, Christopher K.;Jones, A. K. Barney;Taylor, Nick;Cramer, Rainer
关键词：
牛结核病（bTB）的检测主要依靠结核菌素皮肤试验，需要两次单独的动物处理事件，其间有一段潜伏期（通常为3天）。在这里，我们提出使用液体大气压(LAP)-MALDI来识别bTB感染，采用由监督线性判别分析（LDA）获得的三级预测模型，并以牛乳腺炎样本作为疾病阳性对照进行测试。采用无创收集鼻拭子收集样本，随后进行短时间（<4小时）的样品制备方法。对经过处理的鼻拭子的三级LDA模型进行交叉验证，灵敏度为75.0%，特异性为90.1%，总体分类准确率为85.7%。这些值与皮肤试验的值相当，表明LAP-MALDI MS有潜力提供一种替代的单次就诊诊断平台，可以在采样当天检测出bTB。
Detecting bovine tuberculosis (bTB) primarily relies on the tuberculin skin test, requiring two separate animal handling events with a period of incubation time (normally 3 days) between them. Here, we present the use of liquid atmospheric pressure (LAP)-MALDI for the identification of bTB infection, employing a three-class prediction model that was obtained by supervised linear discriminant analysis (LDA) and tested with bovine mastitis samples as disease-positive controls. Noninvasive collection of nasal swabs was used to collect samples, which were subsequently subjected to a short (<4 h) sample preparation method. Cross-validation of the three-class LDA model from the processed nasal swabs provided a sensitivity of 75.0% and specificity of 90.1%, with an overall classification accuracy of 85.7%. These values are comparable to those for the skin test, showing that LAP-MALDI MS has the potential to provide an alternative single-visit diagnostic platform that can detect bTB within the same day of sampling.
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DOI：
10.1136/vr.102961
发表时间：
2015-09-12
期刊：
The Veterinary record
影响因子：
--
作者：
Goodchild AV;Downs SH;Upton P;Wood JL;de la Rua-Domenech R
通讯作者：
de la Rua-Domenech R
影响因子：
16.6
作者：
Cramer, Rainer;Pirkl, Alexander;Hillenkamp, Franz;Dreisewerd, Klaus
通讯作者：
Dreisewerd, Klaus
影响因子：
8.4
作者：
Piras C;Hale OJ;Reynolds CK;Jones AKB;Taylor N;Morris M;Cramer R
通讯作者：
Cramer R
DOI：
10.1101/pdb.prot4550
发表时间：
2006-10-01
期刊：
CSH protocols
影响因子：
--
作者：
Simpson, Richard J
通讯作者：
Simpson, Richard J
影响因子：
3.3
作者：
MONAGHAN, ML;DOHERTY, ML;QUINN, PJ
通讯作者：
QUINN, PJ